Objective: To develop an HPLC method for simultaneous determination of four iridoid glycosides (geniposide

shanzhiside

deacetyl asperulosidic acid methyl ester and genipin-1-β-D-gentiobioside) in Yinzhihuang granules. Method: Chromatographic conditions were as follows:Kromasil C18 column (4.6 mm×250 mm

5 μm)

mobile phase of acetonitrile(A)-0.05% trifluoroacetic acid solution(B) with gradient elution program(0-15 min

5%-6%A;15-23 min

6%-10%A;23-35 min

10%-22%A)

flow rate 1.0 mL·min-1

detection wavelength 238 nm

column temperature at 30℃. Result: The linear ranges of geniposide

shanzhiside

deacetyl asperulosidic acid methyl ester and genipin-1-β-D-gentiobioside were 0.334 4-3.344 0

0.041 5-0.415 2

0.046 6-0.466 4

0.093 1-0.931 2 μg

respectively;Average recoveries of these four ingredients were 97.3%(RSD 1.6%)

99.6%(RSD 2.7%)

97.6%(RSD 2.3%) and 99.2%(RSD 2.2%)

respectively. Conclusion: This method was convenient and accurate

it could be used to improve quality standard of Yinzhihuang granules.