TANG Wei-hong, WEI Jian-hua, MA Yun-ting. Determination of Buddleoside in Buddlejae Flos Formula Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 50-51.
DOI:
TANG Wei-hong, WEI Jian-hua, MA Yun-ting. Determination of Buddleoside in Buddlejae Flos Formula Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 50-51. DOI: 10.11653/syfj2013200050.
Determination of Buddleoside in Buddlejae Flos Formula Granules by HPLC
Objective: To establish a method for determining buddleoside in Buddlejae Flos formula granules. Method: Buddleoside was separated on Inertsil ODS3C18 column(4.6 mm×250 mm
5 μm) with methanol-0.1% phosphoric acid(55:45) as mobile phase
flow rate was 1.0 mL·min-1
column temperature was at 20℃
detection wavelength was 327 nm
injection volume was 10 μL. Result: Buddleoside had good linear relationship within the range of 0.104 8-1.048 μg with r=0.999 6
the average recovery was 102.2% with RSD 1.9%. Conclusion: This method was simple
accurate and reproducible
it could be used to determine the content of buddleoside in Buddlejae Flos formula granules.