Determination of Triptoquinone B in Tripterygium Glycosides by LC-MS Analysis and HPLC

FU Hui-zheng; ZHONG Rui-jian; LUO Yue-hua; WANG Dong; ZHOU Guo-ping; ZHANG Dong-ming; WAN Kai-hua

doi:10.11653/syfj2013200084

您当前的位置：

首页 >

文章列表页 >

Determination of Triptoquinone B in Tripterygium Glycosides by LC-MS Analysis and HPLC

更新时间：2024-01-04

- Determination of Triptoquinone B in Tripterygium Glycosides by LC-MS Analysis and HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 20, Pages: 84-87(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013200084
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
FU Hui-zheng, ZHONG Rui-jian, LUO Yue-hua, et al. Determination of Triptoquinone B in Tripterygium Glycosides by LC-MS Analysis and HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 84-87.
DOI：

FU Hui-zheng, ZHONG Rui-jian, LUO Yue-hua, et al. Determination of Triptoquinone B in Tripterygium Glycosides by LC-MS Analysis and HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 84-87. DOI： 10.11653/syfj2013200084.

摘要

目的: 建立雷公藤多苷中雷公藤对醌B定性分析和含量测定方法。方法: 利用高效液相色谱-电喷雾-质谱(HPLC-ESI-MS)联用结合LC-DAD技术

通过和标准化合物雷公藤对醌B的保留时间、紫外吸收光谱以及质谱中的准分子离子峰[M+Na]+相对照

快速鉴定雷公藤多苷中的雷公藤对醌B

对其含量的测定则采用了高效液相色谱方法。结果: 一级质谱扫描结果表明雷公藤多苷中保留时间tR=13.2 min的峰化合物结构为雷公藤对醌B

准分子离子峰[M+Na]+为353.1

高效液相色谱结果表明雷公藤多苷中雷公藤对醌B在0.115~1.15 μg呈良好的线性关系(r=0.999 9)

平均回收率和RSD分别为99.32%

0.7%。结论: 该方法简便、准确

可作为雷公藤多苷中雷公藤对醌B的含量测定方法。

Abstract

Objective: To develop an analytic method for determination of triptoquinone B in tripterygium glycosides. Method: The HPLC-ESI-MS and HPLC-DAD methods were established. Triptoquinone B in tripterygium glycosides was quickly identified by comparing the retention time

UV absorption spectrum and quasi-molecular ion peak [M+Na]+ with the reference compound. The content of triptoquinone B in tripterygium glycosides was determined by HPLC. Result: One level mass spectrometry scanning results showed that the chromatographic peak of retention time tR=13.2 min was identified as triptoquinone B

and its quasi-molecular ion peak [M+Na]+ was 353.1.The calibration curves of triptoquinone B was linear between 0.115-1.15 μg (r=0.999 9). The average recovery and the relative standard deviation were 99.32% and 0.7% respectively. Conclusion: The method is simple

accurate and could be used for determination of triptoquinone B in tripterygium glycosides.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

RP-HPLC Determination of Morin-3-O-α-L-lyxopyranoside and Morin-3-O-α-L-arabopyranoside from leaf

Assay of Quercetin in

RP-HPLC Determination of Kaempferide in Fuyang Tablets

Mechanism of Xiaoyaosan in Preventing and Treating Hepatic Injury Caused by Tripterygium Glycosides

Related Author

FU Hui-zheng1

WAN Kai-hua2

LUO Yue-hua2

WAN Kai-hua1

FU Hui-zheng2

LUO Yong-ming2

ZHANG Dong-ming3

LIU Yan

Related Institution

江西省食品药品检验所

1),WAN Kai-hua~2,LUO Yue-hua~2

Integrated Traditional Chinese and Western Medicine， Bethune International Peace Hospital， People's Liberation Army

Hebei University of Chinese Medicine

Gansu Provincial Hospital

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰