Simultaneous Determination of Chlorogenic Acid and Buddleoside in Chrysanthemi Indici Flos by UPLC

HAN Yong-cheng; GONG Hai-yan; LIU Wei; CHEN Ning; CUI Yong-xia; WU Ming-xia; WANG Li-guo; RU Fan-shu

doi:10.11653/syfj2013200088

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Simultaneous Determination of Chlorogenic Acid and Buddleoside in Chrysanthemi Indici Flos by UPLC

更新时间：2024-01-04

- Simultaneous Determination of Chlorogenic Acid and Buddleoside in Chrysanthemi Indici Flos by UPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 20, Pages: 88-90(2013)
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- DOI：10.11653/syfj2013200088
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- Published：2013
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HAN Yong-cheng, GONG Hai-yan, LIU Wei, et al. Simultaneous Determination of Chlorogenic Acid and Buddleoside in Chrysanthemi Indici Flos by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 88-90.
DOI：

HAN Yong-cheng, GONG Hai-yan, LIU Wei, et al. Simultaneous Determination of Chlorogenic Acid and Buddleoside in Chrysanthemi Indici Flos by UPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(20): 88-90. DOI： 10.11653/syfj2013200088.

摘要

目的: 建立UPLC同时测定不同产地野菊花中蒙花苷和绿原酸含量的分析方法。方法: 采用超高效液相色谱系统

C18 色谱柱(2.1 mm×50 mm

1.8 μm)

流动相乙腈-0.1%磷酸水

以0.2 mL·min-1的流速进行梯度洗脱

检测波长326 nm

柱温25℃。结果: 蒙花苷在3.0~120.0 mg·L-1线性关系良好(r=1.000 0)

平均回收率99.43%

RSD 1.54%(n=6);绿原酸在0.48~19.2 mg·L-1线性关系良好(r=1.000 0)

平均回收率98.67%

RSD 2.13%(n=6)。结论: UPLC法分析速度快

重复性好

结果准确且高效、简便

可用于野菊花中蒙花苷和绿原酸含量的测定。

Abstract

Objective: To establish a UPLC method for simultaneous determination of buddleoside and chlorogenic acid in Chrysanthemi Indici Flos from different areas. Method: UPLC was used. The chromatographic separation was performed on a C18 (2.1 mm×50 mm

1.8 μm) column with gradient elute. The flow rate was 0.2 mL·min-1. The detection wavelength was set at 326 nm and column temperature was maintained at 25℃. Result: Good linear relationship between the peak area and content of buddleoside and chlorogenic acid was achieved when the amount was within 3.0-120.0

0.48-19.20 mg·L-1;the recoveries were 99.43% and 98.67%;RSDs were 1.54% and 2.13% respectively(n=6). Conclusion: The UPLC analysis is speed

reproducible

accurate

efficient and convenient

which can detect the content of buddleoside and chlorogenic acid in Chrysanthemi Indici Flos.

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Related Author

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Related Institution

Institute of Traditional Chinese Medicine（TCM），Shanghai University of TCM

Yanling DU Renkang Biotechnology Co. Ltd.

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Henan University of Chinese Medicine

Engineering Technology Research Center for Comprehensive Development and Utilization of Authentic Medicinal Materials in Henan Province， Henan University of Chinese Medicine

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