Objective: To establish a mice model of mammary gland hyperplasia

to observe the effects of Ruluotong on estradiol (E2)

progesterone (P)

visceral index

and pathologic changes of breast tissue in mammary gland of the model mice

and to study the mechanism of the actions. Method: Female mice were divided into blank control group

model control group

Ruluotong capsules high

midlle and low doses group (0.6

0.3

0.15 g·kg-1)

Rukang tables group(0.53 g·kg-1). Model of mammary glands hyperplasia in mice was established by taking estradiol benzoate and progesterone. The treatment groups were given with different doses of Ruluotong capsules and Rukang pills for thirty days. The contents of E2

P of serum and visceral index were determined

pathologic changes of breast tissue were observed. Result: Ruluotong high

medlle and low doses could significantly decrease E2 level(P<0.01) of model serum and increase the P content of serum(P<0.05). Thymus index in the high dose group was markedly(P<0.01)and womb index was significantly increased(P<0.01). Pathomorphological investigation showed three doses of Ruluotong could significantly reduce lobular breast density and alveolar surface area(P<0.01)

and increase lobular interstitial density(P<0.01). Pathological changes were mitigated by the capsules

with inhibition of mammary lobular hyperplasia

showing shrink in lobular size

narrowed acinar and duct. Conclusion: Ruluotong capsules can improve the pathological changes of the mammary gland hyperplasia by regulating proliferation of breast estrogen levels in mice to inhibit mammary gland hyperplasia of mice induced by estrogen effectively.