ZHANG Ying, HAO Ying, YANG Li-man, et al. Effects of Different Steaming Processes on Ginsenosides from Ginseng Radix et Rhizoma Rubra[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 16-20.
DOI:
ZHANG Ying, HAO Ying, YANG Li-man, et al. Effects of Different Steaming Processes on Ginsenosides from Ginseng Radix et Rhizoma Rubra[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 16-20. DOI: 10.11653/syfj2013210016.
Effects of Different Steaming Processes on Ginsenosides from Ginseng Radix et Rhizoma Rubra
进样量5 μL;通过高分离度液相色谱-四极杆-飞行时间质谱比较不同蒸制温度和烘干温度对人参皂苷类成分种类的影响。 结果: 人参总皂苷含量最高的炮制工艺为升温时间60 min
蒸制温度100 ℃
蒸制时间6 h
烘干温度50 ℃
蒸制温度和烘干温度对总皂苷含量具有显著性影响。不同蒸制温度和烘干温度下
人参皂苷Rb1
Rb2
Rc
Rd
Re
Rg1
Rg2
Rg3
Rh1
Rh2 含量均有显著变化。 结论: 实际生产中应根据需求不同
调整红参加工方法
实现质量控制
为人参皂苷类成分的工业化生产和新药开发提供参考。
Abstract
Objective: To investigate effects of temperature
time in processing process of Ginseng Radix et Rhizoma Rubra on the content of ginsenosides. Method: The content of total saponins was determined by UV
orthogonal test was adopted to investigated effects of heating time
steaming temperature
steaming time
drying temperature on total saponins content.The contents of ginsenoside Rb1
Rb2
Rc
Rd
Re
Rg1
Rg2
Rg3
Rh1
Rh2 were proformed by HPLC
chromatographic conditions were as follows:ZORBAX SB-C18 column(2.1 mm×100 mm
3.5 μm)
column temperature 30 ℃
mobile phase of water-acetonitrile gradient elution
flow rate 1.0 mL·min-1
detection wavelength 203 nm
injection volume 5 μL;Effects of different steaming and drying temperature on kinds of ginsenosides were compared by high resolution liquid chromatography-quadrupole-time of flight mass spectrometry. Result: Optimum processing technology for the highest content of total saponins was:temperature raised to 100 ℃ at 60 min
steamed for 6 h and dried at 50 ℃
steaming and drying temperature had significant difference.Under different steaming and drying temperature
the contents of ginsenoside Rb1
Rb2
Rc
Rd
Re
Rg1
Rg2
Rg3
Rh1
Rh2 changed remarkably. Conclusion: According to needs of actual production
processing method of Ginseng Radix et Rhizoma Rubra should adjust for quality control.This study would provide a reference for production and new drugs development of ginsenosides.
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Related Author
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HU Longxiao
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Related Institution
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