Optimization of Extraction Process for Gallic Acid in  from Guizhou by Orthogonal Test

WEI Xue-jun; Chen Ming; YU Yue-sheng; LIN Xian-yan; FENG Guang-wei; LI Jiang

doi:10.11653/syfj2013210027

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Optimization of Extraction Process for Gallic Acid in from Guizhou by Orthogonal Test

更新时间：2024-01-04

- Optimization of Extraction Process for Gallic Acid in from Guizhou by Orthogonal Test
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 21, Pages: 27-29(2013)
- 作者机构：
- 作者简介：
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- DOI：10.11653/syfj2013210027
  CLC：
- Published：2013
- 稿件说明：
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WEI Xue-jun, Chen Ming, YU Yue-sheng, et al. Optimization of Extraction Process for Gallic Acid in from Guizhou by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 27-29.
DOI：

WEI Xue-jun, Chen Ming, YU Yue-sheng, et al. Optimization of Extraction Process for Gallic Acid in from Guizhou by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 27-29. DOI： 10.11653/syfj2013210027.

摘要

目的: 优选黔产铁苋菜中没食子酸的提取工艺。方法: 采用HPLC测定铁苋菜中没食子酸含量

Diamonsil C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-0.05% 磷酸(4:96)

检测波长270 nm

柱温室温

流速1 mL·min-1

进样量10 μL。以没食子酸转移率为指标

通过单因素试验筛选提取溶媒和提取方法

采用正交试验考察液固比、乙醇体积分数、提取温度、提取时间对提取工艺的影响。结果: 选择乙醇为提取溶媒

采用回流法提取。最佳提取工艺条件为加30倍量70% 乙醇于90 ℃提取2次

每次4 h

没食子酸平均转移率7.888 mg·g-1(RSD 1.60%)。结论: 优选的提取工艺稳定可行

为黔产铁苋菜的开发利用提供实验依据。

Abstract

Objective: To optimize extraction process of gallic acid in Acalypha australis from Guizhou. Method: The content of gallic acid from A. australis was determined by HPLC

Diamonsil C18 column(4.6 mm×250 mm

5 μm)

mobile phase of acetonitrile-0.05% phosphoric acid(4:96)

detection wavelength 270 nm

column temperature at room temperature

flow rate 1 mL·min-1

injection volume 10 μL.With transfer rate of gallic acid as index

single factor tests were adopted to screen extraction methods and solvents

effects of ratio of solid-liquid

ethanol concentration

extraction temperature and time on extraction technology were investigated by orthogonal test. Result: The best extraction process of gallic acid was as following:extracted 2 times with 30 times the amount of 70% ethanol at 90 ℃

4 hours per time.Average transfer rate of gallic acid was 7.888 mg·g-1(RSD 1.60%). Conclusion: This optimized extraction process was stable and feasible

it could provide experimental basis for development and utilization of A. australis from Guizhou.

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Related Author

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DU Shu-shan

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FEI Chao

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Related Institution

Department of Pharmacy,Affiliated Hospital of Medical College of Chinese People’s Armed Police Forces

2 Protection and Utilization of Traditional Chinese Medicine of Beijing Area Major Laboratory,Beijing Normal University

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

上海中医药大学中药现代制剂技术教育部工程研究中心

Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine

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