Quality Evaluation of Wildlife Tending  in Yunnan Province

DUAN Bao-zhong; HUANG Lin-fang; SHANG Fei-neng; CAO Chang-e; ZHENG Si-hao

doi:10.11653/syfj2013210082

您当前的位置：

首页 >

文章列表页 >

Quality Evaluation of Wildlife Tending in Yunnan Province

更新时间：2024-01-04

- Quality Evaluation of Wildlife Tending in Yunnan Province
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 21, Pages: 82-86(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013210082
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
DUAN Bao-zhong, HUANG Lin-fang, SHANG Fei-neng, et al. Quality Evaluation of Wildlife Tending in Yunnan Province[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 82-86.
DOI：

DUAN Bao-zhong, HUANG Lin-fang, SHANG Fei-neng, et al. Quality Evaluation of Wildlife Tending in Yunnan Province[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 82-86. DOI： 10.11653/syfj2013210082.

摘要

目的: 研究野生抚育粗茎秦艽药材的有效成分和化学指纹图谱

以期评价其质量

为粗茎秦艽药材的GAP种植提供科学依据。方法: 采用Zorbax SB C18(4.6 mm×250 mm

5 μm)色谱柱

流动相甲醇-水

梯度洗脱

流速1 mL·min-1

检测波长238 nm

柱温为25 ℃。利用国家药典委员会出版的《中药色谱指纹图谱相似度评价系统》2004 A版软件

对15批3年生野生抚育粗茎秦艽药材进行指纹图谱分析。结果: 15批粗茎秦艽药材环烯醚萜苷类有效成分及浸出物含量符合2010年版《中华人民共和国药典》要求

各样地药材化学指纹色谱相似度>0.981

质量均一性好。结论: 方法具有良好的精密度、重复性、稳定性

可用于粗茎秦艽药材的质量综合评价。野生抚育模式药材可作为粗茎秦艽主要种植发展模式

对云南建设"云药之乡"和发展道地药材具有重要的现实意义。

Abstract

Objective: Radix Gentiana crassicaulis is the major cultivated Gentiana species in Yunnan Province. Wildlife tending is a major cropping pattern. In this study

an improved and comprehensive HPLC fingerprints were developed for the quality evaluation of G. crassicaulis

together with the simultaneous determination of four iridoid and secoiridoid glycosides. Loganic acid

swertiamarin

gentiopicroside

sweroside were also detected. Method: HPLC separation was performed on an Agilent Zorbax SB C18 column. The mobile phase was methanol and water with linear gradient change of methanol from 0 to 40% in 20 min. The flow rate was 1.0 mL·min-1

and the detection wavelength was set at 238 nm. Result: The HPLC chromatograms of fifteen samples from different regions of China showed a similar pattern. Thirteen peaks were selected as the characteristic peaks to evaluate the similarities among different G. crassicaulis samples. Conclusion: This is the first report on an HPLC method for the simultaneous determination of four bioactive components and fingerprint analysis of wildlife tending G. crassicaulis. The results indicate that this comprehensive method is suitable for evaluate the quality of G. crassicaulis for GAP planting.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Herbal Textual Research on Genuine Medicinal Materials " Deng Ju" and Quality Evaluation of Nan Yang Chrysanthemi Flos

HPLC Fingerprint of Bletillae Formosanae Rhizoma

HPLC Fingerprint of Different Polarity Fractions of Gardeniae Fructus with Different Fruit Shapes

Study on HPLC Fingerprint of Poria Grew in the Different Places

Study on Quality Evaluation of Hawthorn

Related Author

Peng-fei HAO

Si-yuan ZHANG

Zhi-hui DU

Hua BIAN

Xian-zhang HUANG

Mei WU

Hong-zhe LI

Yong-mei XUE

Related Institution

Nanyang Institute of Technology, Zhang Zhongjing College of Chinese Medicine

Nanyang Institute of Technology, Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation

College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine

Henan University of Chinese Medicine

Zhongjing Wanxi Pharmaceutical Co. Ltd.

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰