Objective: To develop an HPLC-ELSD method for determination of the content of pinocembrin

cardamonin

sipeimine and sipeimine-3β-D-glucoside in Houzao Ninhuangsan(HZNHS). Method: An Hypersil C18 column was used as the chromatographic column

the flow rate was 0.9 mL·min-1. The mobile phase for pinocembrin and cardamonin consisted of methanol (A) and 0.5% phosphoric acid solution (B);the UV detection wavelength was at 300 nm.The mobile phase for sipeimine and sipeimine-3β-D-glucoside consisted of acetonitrile-water-diethylamine (65:35:0.5). The temperature of drift tube was set at 80 ℃

and the gas flow (N2) was set at 2.3 SLPM·min-1. Result: There was a good linear relationship between the concentration of pinocembrin

cardamonin and peak area value when the concentrations of pinocembrin and cardamonin were within the range of 0.052 7-1.054 μg (r=0.999 4)

0.036 2-0.724 0 μg (r=0.999 8). The average recovery were 97.14% (RSD 1.10%) and 97.64% (RSD 1.39%). There was a good linear relationship when the concentrations of sipeimine and sipeimine-3β-D-glucoside were within the range of 0.057 1-1.142 μg (r=0.999 1)

0.081 3-1.626 μg (r=0.999 5). The average recovery was 97.55% (RSD 1.75%) and 98.09% (RSD 1.80%). Conclusion: The method was accurate

sensitive

reproducible and may be used in the determination of pinocembrin

cardamonin

sipeimine and sipeimine-3β-D-glucoside in HZNHS.