Objective: To observe the effect of Lurong Jiangu dripping pills on the bone mineral density (BMD) of femur and expression of TRPV5 in kidney of rats with glucocorticoid-induced osteoporosis (GIO)

explore the mechanism of GIO. Method: Eighty-two Wistar rats were randomly divided into normal group(A)

model group(B)

Lurong Jiangu dripping pills group(C) and Gushukang group(D). By intramuscular injection of dexamethasone(2.5 mg·kg-1)twice a week rat models was established. The treatment lasted for 9 weeks BMD of femur in vitro was detected with dual-energy X-ray absorptiometry

the mRNA and protein expression of TRPV5 in kidney were detected by real-time quantitative RT-PCR and Western blot methods. Result: Compared with the normal group

the BMD of model group rats decreased evidently(P<0.01)

mRNA and protein expression of TRPV5 in kidney of model group rats decreased(P<0.01). Compared with the model group

the BMD of Lurong Jiangu dripping pills group increased(P<0.01)

mRNA and protein expression of TRPV5 also increased(P<0.01). Conclusion: The depressant effect of dexamethasone for the calcium transport in kidney was the main pathomechanism of GIO.Lurong Jiangu dripping pills can upregulate the mRNA and protein expression of TRPV5 in kidney to promote renal calcium reabsorption.