Effects of Hei Xiaoyao San on SOD, GSH-Px and MDA of Brain Tissue and Serum in Alzheimer’s Disease Rat Model Induced by A Fragments

LI Hai-long; WANG Hu-ping; LIU Jian-hong; GU Jing; CHE Min; LAN Mei-hua; WU Hong-yan

doi:10.11653/syfj2013210186

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Effects of Hei Xiaoyao San on SOD, GSH-Px and MDA of Brain Tissue and Serum in Alzheimer’s Disease Rat Model Induced by A Fragments

更新时间：2024-01-04

- Effects of Hei Xiaoyao San on SOD, GSH-Px and MDA of Brain Tissue and Serum in Alzheimer’s Disease Rat Model Induced by A Fragments
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 21, Pages: 186-189(2013)
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- DOI：10.11653/syfj2013210186
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- Published：2013
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LI Hai-long, WANG Hu-ping, LIU Jian-hong, et al. Effects of Hei Xiaoyao San on SOD, GSH-Px and MDA of Brain Tissue and Serum in Alzheimer’s Disease Rat Model Induced by A Fragments[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 186-189.
DOI：

LI Hai-long, WANG Hu-ping, LIU Jian-hong, et al. Effects of Hei Xiaoyao San on SOD, GSH-Px and MDA of Brain Tissue and Serum in Alzheimer’s Disease Rat Model Induced by A Fragments[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 186-189. DOI： 10.11653/syfj2013210186.

摘要

目的: 观察黑逍遥散对β淀粉样蛋白(Aβ25-35)诱导阿尔芡海默病(AD)模型大鼠脑组织和血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及丙二醛(MDA)的影响

探讨黑逍遥散防治AD的作用及其机制。方法: 雄性SPF级SD大鼠110只

先按随机数字表挑选16只大鼠作为假手术组

其余大鼠手术7 d后进行水迷宫测试

将测试合格的大鼠再按照随机数字表法分为5组:模型组、阳性对照组、黑逍遥散高、中、低剂量组

每组14只。假手术组14只。采用Aβ25-35海马区注射诱发大鼠脑内神经元损伤

复制大鼠AD模型;造模后7 d

黑逍遥散各组大鼠分别按低、中、高剂量(4.2

8.50

17.0 g·kg-1·d-1)ig给药

阳性对照组给予石杉碱甲片溶液0.02 mg·kg-1·d ig

模型组、假手术组ig给予等容量双蒸水

每日上午1次

连续治疗28 d。末次给药后处死大鼠并采集血清和脑标本

比色法测大鼠血清和脑匀浆SOD

MDA

GSH-Px活性; 结果: 与假手术组比较

模型组大鼠血清和脑组织匀浆中的SOD

GSH-Px显著降低

MDA含量显著增高;与模型组比较

黑逍遥散能显著升高Aβ25-35诱导AD模型大鼠血清和脑组织中SOD 与GSH-Px活性、降低MDA含量(P<0.05)。结论: 黑逍遥散通过抗氧化作用起到保护AD 模型大鼠的作用。

Abstract

Objective: To explore the influence of Hei Xiaoyao San on superoxide dismutase(SOD)

gluthathione peroxidase(GSH-Px) and malondialdehyde(MDA) of brain tissue and serum in Alzheimer's disease rat model induced by β-amyloid protein(Aβ25-35) fragments so as to discuss the role and mechanisms involved in Hei Xiaoyao San in the prevention and treatment of Alzheimer's disease(AD). Method: The SD rats (n=94) were randomly divided into sham surgery rats(n=16) group and model group (n=94)

and all rats of model group received water maze tests for 7 days. These eligible rats were randomly divided 5 groups

model group

positive control group

Hei Xiaoyao San high

medium and low dose groups

14 rats in each group. Aβ25-35fragments was injected into the hippocampus to damage brain neuron to make the rat's AD model. 7 days later

all rats of Hei Xiaoyao San group were orally administered Hei Xiaoyao San at low

medium and high doses (4.2

8.50

17.0 g·kg-1·d-1)

the positive control group was orally given huperzine A solution 0.02 mg·kg-1·d

model group and sham group received an equal volume double distilled water daily for 28 d. After the last administration

the rats were sacrificed and serum and brain samples collected

and the serum and brain homogenate stration of SOD

MDA

GSH-Px were examined by means of chemistry chromatometry. Result: Compared with the sham group

serum and brain tissue homogenate SOD

GSH-Px of model group decreased significantly

MDA content increased significantly(P<0.05). Compared with the model group

Hei Xiaoyao San obviously increased the activities of SOD and GSH-Px and reduced the content of MDA (P<0.05) in serum and brain tissue. Conclusion: Hei Xiaoyao San can protect rat's AD model from damage

and the mechanisms of which may be related to anti-free radicals.

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