Objective: The aim of this study was to investigate the effects of Zhengan Xifeng decoction on the spontaneous movement in 1-methy-4-phenyl-1

2

3

6-tetrahy-dropyridine(MPTP)-treated C57BL/6 mice

and some mechanisms involved. Method: C57BL/6 mice were given MPTP (30 mg·kg-1) to prepare Parkinson disease mice model. Different doses of Zhengan Xifeng decoction (12.5

25

50 g·kg-1)

or selegiline (10 mg·kg-1) were given for 14 d in the pretreatment groups. The behavioral analysis of C57BL/6 mice was performed by using spontaneous movement test. Real-time PCR and Western blot were used to analyze the expression of 14-3-3 and tyrosine hydroxylase (TH) mRNA and protein. Result: Zhengan Xifeng decoction could reduce the number of spontaneous movement of MPTP-treated C57BL/6 mice. Zhengan Xifeng decoction could also up-regulate the expression of 14-3-3 and TH mRNA and protein. Conclusion: Anti-Parkinson disease effect of Zhengan Xifeng decoction may be attributable to up-regulation mRNA and protein expression of 14-3-3 and TH in MPTP-treated C57BL/6 mice.