Effect of Liuwei Buqi Capsule on Expression of STAT4, STAT6 Protein in Rats Model with FQDS of COPD

WANG Cheng-yang; LI Ze-geng; LIU Xiang-guo; PENG Qing-he; WU Song; ZHANG Kai; LV Lei

doi:10.11653/syfj2013210194

您当前的位置：

首页 >

文章列表页 >

Effect of Liuwei Buqi Capsule on Expression of STAT4, STAT6 Protein in Rats Model with FQDS of COPD

更新时间：2024-01-04

- Effect of Liuwei Buqi Capsule on Expression of STAT4, STAT6 Protein in Rats Model with FQDS of COPD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 21, Pages: 194-198(2013)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.11653/syfj2013210194
  CLC：
- Published：2013
- 稿件说明：
移动端阅览
WANG Cheng-yang, LI Ze-geng, LIU Xiang-guo, et al. Effect of Liuwei Buqi Capsule on Expression of STAT4, STAT6 Protein in Rats Model with FQDS of COPD[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 194-198.
DOI：

WANG Cheng-yang, LI Ze-geng, LIU Xiang-guo, et al. Effect of Liuwei Buqi Capsule on Expression of STAT4, STAT6 Protein in Rats Model with FQDS of COPD[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 194-198. DOI： 10.11653/syfj2013210194.

摘要

目的: 观察六味补气胶囊对慢性阻塞性肺疾病(COPD)肺气虚证模型大鼠细胞信号转导转录因子(STAT)4

STAT6蛋白表达影响及机制。方法: 将大鼠随机分为正常组、模型组、六味补气胶囊高剂量组(六高组)、六味补气胶囊低剂量组(六低组)、金水宝胶囊组(中药对照)、脾氨肽组(西药对照)

每组12只。除正常组外

其余大鼠采用烟熏加脂多糖(LPS)气管滴入方法建立COPD肺气虚证大鼠模型

模型复制成功第28天给药

各组给药剂量为:正常组、模型组:生理盐水0.09 g·kg-1;六高组:六味补气胶囊0.16 g·kg-1;六低组:六味补气胶囊0.04 g·kg-1;金水宝组:金水宝49.5 mg·kg-1

脾氨肽组:灌服脾氨肽0.033 mg·kg-1

1次/d

连续给药14 d。观察各组大鼠肺组织STAT4

STAT6蛋白表达变化。结果: 与正常组比较

STAT4蛋白在COPD肺气虚证大鼠肺组织中表达的阳性面积增加、平均光密度增强 (P<0.01)

而STAT6蛋白表达的阳性面积减小、平均光密度降低(P<0.01);经六味补气胶囊等药治疗后

较之模型组

各治疗组STAT4的表达面积减小、强度减弱

STAT6的表达面积扩大(P<0.05)

各治疗组之间无明显差异。结论: 六味补气胶囊可抑制COPD肺气虚证大鼠肺组织中STAT4蛋白表达

促进STAT6蛋白的表达。

Abstract

Objective: To observe the influence of Liuwei Buqi capsule(LBC) on the expression of STAT4 and STAT6 protein and to study the therapeutic effects and possible mechanism in rat model with Fei-qi deficiency syndrome(FQDS) of chronic obstructive pulmonary disease(COPD). Method: The rats were randomly divided into normal group

model group

high dose of Liuwei Buqi group (0.16 g·kg-1)

low dose of Liuwei Buqi capsule group (0.04 g·kg-1)

Jinshuibao capsule group(49.5 mg·kg-1)

spleen aminopeptidase group(0.033 mg·kg-1)

(n=12). The rat model of lung deficiency of COPD was established by smoking rats and dripping the LPS into the trachea. At twenty-eight days after the modeling. corresponding drugs were given.The expression of STAT4 and STAT6 protein of lung tissue was observed. Result: Compared with the normal control group

the average optical density of STAT4 protein in lung tissue and the positive area in model group increased markedly

STAT6 protein decreased (P<0.01).Compared with the model group

the positive area of STAT4 became smaller and the average optical density became weaker

the positive area of STAT6 increased;meanwhile

no statistical significance was found among the treatment groups. Conclusion: The LBC can inhibit the expression of STAT4 protein

and promote the expression of STAT6 protein.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Combination Rules of Chinese Herb Prescriptions for Treating Chronic Obstructive Lung Disease by Complex Network

Related Author

HUANG Yuan

YANG Ming

CHEN Jia-lei

XUE Hong-hao

ZHANG Hui-yong

HUANG Yuan1

YANG Ming2

CHEN Jia-lei2

Related Institution

Xinhua Hospital Affiliated to College of Medicine,Shanghai Jiaotong University

Longhua Hospital Affiliated to Shanghai Univesity of Traditional Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰