Antioxidant Effect of Each Part of Ethanol Extrac of

ZHAO Lei; HUANG Shan; JIN Yan; PEI Xiao-na; XU Dan

doi:10.11653/syfj2013210210

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Antioxidant Effect of Each Part of Ethanol Extrac of

更新时间：2024-01-04

- Antioxidant Effect of Each Part of Ethanol Extrac of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 21, Pages: 210-213(2013)
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- DOI：10.11653/syfj2013210210
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- Published：2013
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ZHAO Lei, HUANG Shan, JIN Yan, et al. Antioxidant Effect of Each Part of Ethanol Extrac of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 210-213.
DOI：

ZHAO Lei, HUANG Shan, JIN Yan, et al. Antioxidant Effect of Each Part of Ethanol Extrac of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 210-213. DOI： 10.11653/syfj2013210210.

摘要

目的: 通过体外还原力和抗氧化评价方法研究藏药松生等提取物的抗氧化活性。方法: 将松生等用70% 乙醇浸泡、提取、浓缩所得浸膏依次用石油醚

二氯甲烷

乙酸乙酯

正丁醇、乙醇进行萃取

各组分都配制成适当稀释倍数的溶液

通过普鲁士蓝法、Fenton法和

1-二苯基-2-三硝基苯肼(DPPH)法分别测定各组分的还原力

·OH自由基的清除能力以及抗氧化的活性

并计算各组分的IC50。结果: 3种抗氧化实验表明乙酸乙酯、正丁醇、乙醇部分都具有明显抗氧化的作用。其中用DPPH法测得的IC50分别是乙酸乙酯0.060 5 g·L-1

正丁醇0.076 3 g·L-1

乙醇0.083 3 g·L-1。结论: 提取物清除率与样品浓度存在明显的量效关系

浓度大于1.0 g·L-1时其还原能力几乎接近于维生素(VC)

清除能力接近甚至超过60%。其中在大极性溶液层还原力和抗氧化的能力最强。

Abstract

Objective: To study the scavenging activity in vitro of each part of ethanol extract of Rhamnella gilgitica Mansfeild et Melch. Method: R. gilgitica was extracted with 70% ethanol

then was extracted by petroleum ether

dichloromethane

ethyl acetate

n-butyl alcohol

ethanol the each part was configured to the appropriate solution

Prussian blue method Fenton method and 2

2-diphenyl-1-picrylhydrozyl(DPPH) were used to measure the antioxidantactivity. Result: The ethyl acetate

n-butyl alcohol and ethanol extract of R. gilgitica showed obvious scavenging effects

IC50 of the ethyl acetate

n-butyl alcohol and ethanol extract of R.gilgitica with DPPH was 0.060 5

0.076 3

0.083 3 g·L-1

respectively. Conclusion: The scavenging activity showed a dose-response relationship

even reach 60% by the above 1.0 g·L-1concentrations.The high polarity of solvents layer extract has the strongest free radical scavenging activity.

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