Objective: To study the effect and mechanism of Buzhong Yiqi decoction on rats' wound healing

and to achieve the purpose of wound healing from medical treatment of spleen. Method: Sixty SD rats were randomly divided into normal group

spleen deficiency model group and Buzhong Yiqi decoction group (7 g·kg-1)

and each group is divided into two subgroups of postoperative 3 d and 7 d. Model group and Buzhong Yiqi decoction group were fed with Rhubarb (20 g·kg-1) for 18 days

and normal group was given an equal volume of distilled water. From the 19th day

rats of each group were made the surgical wound;Buzhong Yiqi decoction group were administered with Buzhong Yiqi decoction until the date of sacrifice

and other groups given an equal volume of distilled water. After rats were sacrificed

muscle tissue of wound was prepared. Hydroxyproline (Hyp) was measured using alkaline hydrolysis

and vascular endothelial growth factor (VEGF) and α-smooth muscle actin (α-SMA) were detected using Western blot. Result: Compared with the normal group

Hyp

VEGF and α-SMA of model group were significantly decreased (all P<0.01). Compared with model group of postoperative 3 d

Hyp

VEGF and α-SMA of Buzhong Yiqi decoction group of postoperative 3 d were significantly increased (P<0.01

P<0.01

P<0.05)

but much lower than those of normal group of postoperative 3 d (all P<0.05). Compared with model group of postoperative 7 d

Hyp

VEGF and α-SMA of Buzhong Yiqi decoction group of postoperative 7 d were significantly increased (P<0.01

P<0.05

P<0.01)

but there were no statistical difference between Buzhong Yiqi decoction group and normal group. Conclusion: Buzhong Yiqi decoction can effectively promote wound healing by increasing Hyp

VEGF and α-SMA

and extension of administration time is more conducive to wound healing.