Objective: A comparative study of two boiling method (ephedra first boiling and four herbs with boiling) the proceeds Maxin Shigan decoction of influenza A virus infection in mice. Method: Nasal inoculation method to establish the type A influenza virus lung infection in mice model

experimental set normal comparison group

model comparison group

ephedra first boiling group

four herbs with boiling group

oseltamivir group

7 days of continuous given medicines by intragastric. Respectively

4 days and 7 days after infection to detect the body weigh of mice

on the 7th day

routine testing lung weight index;observation of lungchanges by optical microscopy techniques

lung tissues were observed ultrastructure by transmission electron microscopy techniques. Result: ① Body weight:4 days and 7 days after infection

compared with the normalcomparison group

model comparison group significant weight loss(P<0.05)

7 days after therapy

compared with the model comparison group

Oseltamivir group

ephedra first boilinggroup significant weight gain(P<0.01 or P<0.05).② Lung index:compared with the normal comparison group

the mouse lung index of model comparison group increased significantly(P<0.01).Compared with the model comparison group

lung index was significantly reduced in all treatment groups(P<0.01 or P<0.05). The significant difference(P<0.01)about of the ephedra first boiling group compared with the model comparison group is higher than thefour herbs with boiling group compared with the model comparison group on the significance(P<0.05).③Lung pathological changes:compared with the normal comparison group

the mouse lungs normal tissue structure disappeared

the alveolar inflammatory cell infiltration. Compared with the model comparison group

lung tissue of all treatment groups pathological change significantly improvely

among oseltamivir group and ephedra first boiling group

the level of lung disease in mice is lighter than four herbs with boiling group.④ Lung tissue ultrastructure:compared with thenormal comparison group

the mouse lung tissue ultrastructure of model comparison group was damaged

theperformance of the nuclear membrane and the plasma membrane are blurred

reduced organelle

the mitochondrial cristaeis reduce ordisappear

endoplasmic reticulum emerges vacuolar lesions

there are a large number of virus particles in the cytoplasm

each treatment group lung tissue structure has been improved.Among oseltamivir group and ephedra first boiling group

the extent of lung tissue ultrastructure diseasein mice is completer than four herbs with boiling group. Conclusion: The ephedra first boiling decoction is more effective than the four herbs with boilingdecoction on againning type A influenza virus.