Effects of Jiedu Quyu Prescription Combined with Chemotherapy on Expression of Bcl-2/Bax and Apoptosis of MCF-7 Cells

WANG Bin; XIE Guang-ru; PAN Zhan-yu; QIAN Jun-qiang; WANG Dong; JIA Yan-tao

doi:10.11653/syfj2013210236

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Effects of Jiedu Quyu Prescription Combined with Chemotherapy on Expression of Bcl-2/Bax and Apoptosis of MCF-7 Cells

更新时间：2024-01-04

- Effects of Jiedu Quyu Prescription Combined with Chemotherapy on Expression of Bcl-2/Bax and Apoptosis of MCF-7 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 21, Pages: 236-240(2013)
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- DOI：10.11653/syfj2013210236
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- Published：2013
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WANG Bin, XIE Guang-ru, PAN Zhan-yu, et al. Effects of Jiedu Quyu Prescription Combined with Chemotherapy on Expression of Bcl-2/Bax and Apoptosis of MCF-7 Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 236-240.
DOI：

WANG Bin, XIE Guang-ru, PAN Zhan-yu, et al. Effects of Jiedu Quyu Prescription Combined with Chemotherapy on Expression of Bcl-2/Bax and Apoptosis of MCF-7 Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 236-240. DOI： 10.11653/syfj2013210236.

摘要

目的: 观察解毒祛瘀方联合化疗对MCF-7人乳腺癌细胞凋亡和Bcl-2/Bax表达的影响。方法: 采用MTT法测定药物对MCF-7细胞增殖抑制作用;流式细胞术双标法检测细胞凋亡指数;免疫细胞化学检测Bcl-2

Bax的表达状况。结果: 解毒祛瘀方能够抑制MCF-7细胞的增殖

且对时间、药物浓度呈依赖关系;对化疗有增效作用。通过流式细胞术双标法检测发现解毒祛瘀方对诱导早期MCF-7细胞凋亡更有优势;在分子蛋白层面上

解毒祛瘀方可以明显降低MCF-7细胞Bcl-2的表达

且对时间、药物浓度呈依赖关系;Bax表达与对照组比较则无明显差异;Bcl-2/Bax呈下降趋势。结论: 解毒祛瘀方能抑制肿瘤细胞增殖

促进凋亡

并对化疗有增效作用

降低Bcl-2表达是其可能机制之一。

Abstract

Objective: Observe the effects of Jiedu Quyu prescription combined with chemotherapy on expression of Bcl-2/Bax and apoptosis of MCF-7 cells. Method: Use the MTT to determinate the inhibitory action of medicine to MCF-7 cells; flow cytometry to detect the apoptotic index; immunocytochemistry to examine the Bcl-2

Bax expression. Result: Jiedu Quyu prescription can inhibit the MCF-7 cells proliferation

and on time

drug concentration-dependent; of chemotherapy synergies. Flow cytometry find that Jiedu Quyu prescription on inducing apoptosis in MCF-7 cells early advantage; and it also can decreasing the Bcl-2 expression in MCF-7 cells

also depend on time and drug concentration. Conclusion: The Jiedu Quyu prescription displayed its effect of inhibiting and inducing to apoptotic. It may be associated with the down-regulate of the expression of Bcl-2.

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Related Institution

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School of Chinese Material Medica， Beijing University of Chinese Medicine

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