Objective: To study the influence of Euphorbiae Humifusae herb (EHH) on anti-tumor effect and the expression of apoptosis protein in transplanted liver cancer mice. Method: H22 liver cancer cells were inculated in the right axilla to establish solid tumor models. The animal models were randomized into five groups

the model control group

cyclophosphamide (1 mg·kg-1)group

the EHH(66

132

264 mg·kg-1)group. The medication was given continuously for 14 days. The tumors were weighted. SOD activity and MDA content of serum were measured by the colorimetric analysis. The expression of Bcl-2

Bax and Caspase-3 in tumor tissues were determined by immunohistochemistry. Result: The size of tumor in EHH treat groups was significantly smaller than that in model control group(P<0.05). EHH could significantly decrease MDA level and increase SOD level(P<0.05). The expressions of Bcl-2 were decreased

while the expressions of Bax and Caspase-3 were increased in EHH high

middle dore groups(P<0.05). Conclusion: EHH can inhibit the growth of liver cancer cell and its mechanism is probably related to increased antioxidation

decreased Bcl-2 expression and increased Bax and Caspase-3 expression in transplanted liver cancer mice.