DENG Xiang-liang, ZHU Yuan-hong, Wang Qing, et al. Analysis of Anaphylactoid Reactions Caused by TCMIs via Detection of Histamine Release in Primary Mast Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 271-275.
DOI:
DENG Xiang-liang, ZHU Yuan-hong, Wang Qing, et al. Analysis of Anaphylactoid Reactions Caused by TCMIs via Detection of Histamine Release in Primary Mast Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 271-275. DOI: 10.11653/syfj2013210271.
Analysis of Anaphylactoid Reactions Caused by TCMIs via Detection of Histamine Release in Primary Mast Cells
Objective: To investigate the stimulation effect of 19 traditional Chinese medicine injections(TCMIs)on rat peritoneal mast cell degranulation in vitro. Method: The optimal reaction factors were found to detect histamine of release caused by TCMIs. And rat peritoneal mast cells were isolated and purificated as reference described. The cells were then incubated in Tyrode's containing 100 mL·L-1 TCMI or 4 mg·L-1 C48/80 for 1 h. After washing
degranulation was assessed by measuring histamine in cell lysates as previously described
and percentage of degranulated peritoneal mast cell were calculated in a modified method. Result: The optimal reaction time of histamine and O-phthaldialdehyde(OPT) to form a fluorophore is 3 min.The fluorophore is stability during 7.5-30 min after the reaction was terminated. Percentages of histamine release in two calculation methods showed no difference. The mast cells
stimulated by Tan-re-qing
Qing-kai-ling
Shen-mai
Dan-shen
Xue-sai-tong
Shen-fu Shuang-huang-lian
Sheng-mai
Xiang-dan
Re-du-ning
Fu-fang-dang-gui
had higher percentages in histamine release than that in control. But
those stimulated by Mai-luo-ning
Huang-qi
Ai-di
Xing-nao-jing
Huang-qi-duo-tang
Hong-hua
Yan-suan-chuang-xiong-qin
Shen-qi had no difference with the control in degranulation. Conclusion: Most hypersusceptibility caused by TCMIs may be anaphylactoid reactions. It suggests that rat peritoneal mast cell degranulation test by detecting histamine release can be used to evaluate anaphylactoid reactions caused by TCMIs.
Effect of Medicated Serum Prepared with Chang'an Ⅰ Prescription on Sensitized Mast Cell Degranulation
Studies on Degranuation of Rat Peritoneal Mast Cells and RBL-2H3 Cells Caused by Traditional Chinese Medicine Injections
Effects of Total Saponins of on the Number and Degranulation Ratio of Mast Cells and Expression of Tryptase in Synovium of Rats with Adjuvant Arthritis
Network Correlation Analysis Between Components of Shuanghuanglian Injection and Allergy-like Targets
Related Author
Hai-jie JI
Lin LYU
Hao-meng WU
Feng-yun WANG
Xu-dong TANG
Xiang-xue MA
TANG Jia-ming
ZHAO Yuan
Related Institution
Xiyuan Hospital, China Academy of Chinese Medical Sciences
Shanxi Province Academy of Traditional Chinese Medicine
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