ZAN Jun-feng, XU Bin, LIU Jun-feng, et al. ELSD-HPLC Fingerprint of Triterpenes in Different Origin Place of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 65-68.
DOI:
ZAN Jun-feng, XU Bin, LIU Jun-feng, et al. ELSD-HPLC Fingerprint of Triterpenes in Different Origin Place of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(21): 65-68. DOI: 10.11653/syfj201321065.
ELSD-HPLC Fingerprint of Triterpenes in Different Origin Place of
为评价不同产地的茯苓药材的质量品质提供理论依据。 方法: 色谱柱为Kromasil 100-5 C18柱(4.6 mm×250 mm
5 μm)
流动相0.2% 甲酸水溶液(A)-乙腈(B)
采用梯度洗脱法
流速1.0 mL·min-1
柱温30 ℃
蒸发光检测条件:雾化温度40 ℃
压力350 kPa
gain值为7;进样量20 μL。 结果: 建立的指纹图谱中有18个共有峰
方法学考查表明
各主要色谱峰相对保留时间及峰面积的RSD均<3%
峰面积占总峰面积>10% 的共有峰其相对峰面积差值均在±25% 以内
符合指数图谱的技术要求
仪器的精密度良好
且供试品溶液至少12 h内稳定。 结论: 该方法简便快速、准确可靠
可作为评价不同产地茯苓药材质量的有效手段。
Abstract
Objective: Investigation on RP-HPLC-ELSD fingerprint of triterpenes in Poria cocos from different origin place
in order to provide a theory evidence to evaluate the quality of P. cocos from different origin place
and improve the quality of P. cocos. Method: The chromatographic conditions were Kromasil 100-5 C18 column (4.6 mm×250 mm
5 μm)
0.2% methanoic acid (A) and acetonitrile (B) as gradient mobile phases
flow rate being 1.0 mL·min-1
column temperature at 30 ℃
evaporative light-detection conditions:atomization temperature at 40 ℃
pressure of 350 kPa
gain value of 7
and 20 μL sample injection. Result: Fingerprint was established with 18 common peaks and methodology reached the required standard. Conclusion: The method is simple
rapid
accurate
and can be used as an effective means of P. cocos quality evaluation of different origin place.