Optimization of Extraction Technology for Qingzao Jiufei Decoction by Comprehensive Scoring Method

WU Zhen-qi; PING Jing; YU Yan; WANG Si-yuan; WAGN Zi; WANG Wei; ZHANG Ye

doi:10.11653/syfj2013220012

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Optimization of Extraction Technology for Qingzao Jiufei Decoction by Comprehensive Scoring Method

更新时间：2024-01-04

- Optimization of Extraction Technology for Qingzao Jiufei Decoction by Comprehensive Scoring Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 22, Pages: 12-15(2013)
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- DOI：10.11653/syfj2013220012
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- Published：2013
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WU Zhen-qi, PING Jing, YU Yan, et al. Optimization of Extraction Technology for Qingzao Jiufei Decoction by Comprehensive Scoring Method[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 12-15.
DOI：

WU Zhen-qi, PING Jing, YU Yan, et al. Optimization of Extraction Technology for Qingzao Jiufei Decoction by Comprehensive Scoring Method[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 12-15. DOI： 10.11653/syfj2013220012.

摘要

目的: 优选清燥救肺汤的提取工艺。方法: 采用L9(34)正交试验设计

以苦杏仁苷、绿原酸、甘草酸含量及得膏率的综合评分为指标

经综合评分法考察乙醇体积分数、提取时间、提取次数对工艺的影响。利用HPLC测定3种指标成分的含量

色谱条件为Agilent TC-C18色谱柱(4.6 mm×250 mm

5 μm)

检测波长程序(0~50 min

207 nm;50~100 min

237 nm)

柱温30 ℃

流速1.0 mL·min-1

流动相乙腈(A)-0.1%磷酸水溶液(B)梯度洗脱(0~30 min

5%~10%A;30~55 min

10%~20%A;55~80 min

20%~35%A)。结果: 清燥救肺汤的最佳提取工艺为加8倍量70%乙醇提取2次

每次2 h。结论: 优选的提取工艺稳定、合理、可行

有效成分提取效率高。

Abstract

Objective: To optimize extraction technology of Qingzao Jiufei decoction by multi-index comprehensive evaluation method. Method: L9(34) orthogonal design was adopted

with dry extract rate

the contents of amygdalin

chlorogenic acid and glycyrrhizic acid as comprehensive evaluation index

effects of ethanol concentration

decocting time and times on extraction technology were investigated by composite score method.The contents of three index ingredients were determined by HPLC

chromatographic conditions were as follows:Agilent TC-C18 column(4.6 mm×250 mm

5 μm)

detection wavelength program(0-50 min

207 nm;50-100 min

237 nm)

column temperature 30 ℃

flow rate 1.0 mL·min-1

mobile phase of acetonitrile(A)-0.1% phosphoric acid(B) gradient elution(0-30 min

5%-10%A;30-55 min

10%-20%A;55-80 min

20%-35%A). Result: Optimal extraction technology was as following:extracted 2 times with 8 times the amount of 70% ethanol for 2 h per time. Conclusion: This optimized extraction technology was stable

feasible and reasonable with high extraction efficiency of active ingredients.

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