Inhibition of Platelet Aggregation Investigation and Optimization of Extracting Technology of 6,7-Dehydroroyleanone in Roots of  Schang

doi:10.11653/syfj2013220015

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Inhibition of Platelet Aggregation Investigation and Optimization of Extracting Technology of 6,7-Dehydroroyleanone in Roots of Schang

更新时间：2024-01-04

- Inhibition of Platelet Aggregation Investigation and Optimization of Extracting Technology of 6,7-Dehydroroyleanone in Roots of Schang
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 22, Pages: 15-18(2013)
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- DOI：10.11653/syfj2013220015
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- Published：2013
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Inhibition of Platelet Aggregation Investigation and Optimization of Extracting Technology of 6,7-Dehydroroyleanone in Roots of Schang[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 15-18.
DOI：

Inhibition of Platelet Aggregation Investigation and Optimization of Extracting Technology of 6,7-Dehydroroyleanone in Roots of Schang[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 15-18. DOI： 10.11653/syfj2013220015.

摘要

目的: 评价新疆鼠尾草根中6

7-去氢罗列酮的体外抗血小板聚集作用并优选其提取工艺。方法: 应用比浊法测定6

7-去氢罗列酮体外抗血小板聚集作用。在单因素试验基础上

以6

7-去氢罗列酮提取率为指标

通过正交设计法考察提取次数、料液比和提取时间对6

7-去氢罗列酮提取工艺的影响。采用HPLC测定6

7-去氢罗列酮含量

色谱条件为依利特C18色谱柱(4.6 mm×250 mm

5 μm)

流动相甲醇-0.02%甲酸(83:17)

流速1.0 mL·min-1

检测波长330 nm

柱温40 ℃。结果: 6

7-去氢罗列酮高、中剂量组抑制凝血酶诱导的血小板聚集作用与生理盐水组比较均具有极显著性差异

高剂量组在15

60 min内由凝血酶诱导的血小板抑制聚集率分别为90%

75%

66%

64%。最佳提取工艺为加10倍量95%于85 ℃提取3次

每次3 h

新疆鼠尾草根中6

7-去氢罗列酮质量分数0.01%。结论: 新疆鼠尾草根中6

7-去氢罗列酮表现出较强的抗血小板聚集作用

在此活性基础上优选的提取条件稳定可行。

Abstract

Objective: To evaluate anti-platelet aggregation of 6

7-dehydroroyleanone in roots of Salvia deserta and optimize its extraction process. Method: In vitro anti-platelet aggregation of 6

7-dehydroroyleanone was determined by turbidimetry.Based on single factor tests

orthogonal test was adopted to effects of extracting times

solid-liquid ratio

extracting time on extraction technology of 6

7-dehydroroyleanone with yield of 6

7-dehydroroyleanone as index.The content of 6

7-dehydroroyleanone was determined by HPLC with mobile phase of methanol-0.02% formic acid(83: 17)

detection wavelength of 272 nm

column temperature at 40 ℃. Result: By comparing with the saline group

the high and middle dose group of 6

7-dehydroroyleanone could effectively inhibit platelet aggregation induced by thrombin

platelet aggregation inhibition rates of the high dose group of 6

7-dehydroroyleanone within 15

60 min were 90%

75%

66%

64%

respectively.Optimum extraction process was as following:extracted 3 times with ten-fold the amount of 95% ethanol at 85 ℃ for 3 h each time;The content of 6

7-dehydroroyleanone in roots of S. deserta was 0.01%. Conclusion: 6

7-Dehydroroyleanone in roots of S. deserta showed better anti-platelet aggregation effect

optimized extraction technology was stable

reasonable and feasible which based on the activity.

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Related Institution

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