Optimization of Ethanol Extraction Process for Yinchen Siling Granules

ZHAO Jian; PU Qing-rong; CHEN Yu-lan; HUANG Rui; LUO Qun

doi:10.11653/syfj2013220031

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Optimization of Ethanol Extraction Process for Yinchen Siling Granules

更新时间：2024-01-04

- Optimization of Ethanol Extraction Process for Yinchen Siling Granules
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 22, Pages: 31-34(2013)
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- DOI：10.11653/syfj2013220031
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- Published：2013
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ZHAO Jian, PU Qing-rong, CHEN Yu-lan, et al. Optimization of Ethanol Extraction Process for Yinchen Siling Granules[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 31-34.
DOI：

ZHAO Jian, PU Qing-rong, CHEN Yu-lan, et al. Optimization of Ethanol Extraction Process for Yinchen Siling Granules[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 31-34. DOI： 10.11653/syfj2013220031.

摘要

目的: 优选茵陈四苓颗粒的乙醇提取工艺。方法: 以栀子苷提取量为指标

通过单因素试验比较乙醇回流法、乙醇超声法和乙醇渗漉法

确定茵陈四苓颗粒中部分药材的提取方法;通过正交试验考察乙醇体积分数、浸泡时间、加醇量、渗漉流速对提取工艺的影响。采用HPLC测定栀子苷含量

色谱条件为UltimateTMC18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-水(15:85)

流速1 mL·min-1

柱温40 ℃

检测波长238 nm

进样量10 μL。结果: 采用乙醇渗漉法提取

最佳提取工艺条件为加10倍量70%乙醇浸泡12 h

渗漉流速5 mL·min-1

栀子苷提取量达42.524 4 mg·g-1。结论: 该工艺客观可行、稳定合理

可为茵陈四苓颗粒的工业生产提供理论依据。

Abstract

Objective: To optimize ethanol extraction technology of Yinchen Siling granules. Method: With yield of geniposide as index

single factor test was adopted to screen ethanol extraction method

such as refluxing method

ultrasonic method and percolation method;Effects of ethanol concentration

soaking time

ethanol amount and percolation velocity on extraction technology was investigated by orthogonal design.The content of geniposide was determined by HPLC

chromatographic conditions were as follows:UltimateTMC18 column(4.6 mm×250 mm

5 μm)

mobile phase of acetonitrile-water(15: 85)

flow rate 1 mL·min-1

column temperature 40 ℃

detection wavelength 238 nm

injection volume 10 μL. Result: Ethanol percolation method was adopted

the best extraction conditions were as follows:soaked 12 h by adding 10 times the amount of 70% ethanol with percolation velocity of 5 mL·min-1;Under these conditions

yield of geniposide was up to 42.524 4 mg·g-1. Conclusion: This optimized extraction process was stable

reasonable and practical

it could provide a theoretical basis for industrial production of Yinchen Siling granules.

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Related Author

Jing LIU

Xiao HUANG

Xiao-mei FU

Chao YANG

Fang WANG

De-hong LIU

Jian-guo PEI

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Related Institution

School of Pharmacy，Jiangxi University of Traditional Chinese Medicine

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Beijing Zhongyan Tongrentang Pharmaceutical Research and Development Co. Ltd.

School of Life Sciences, Beijing Institute of Technology

Henan University of Chinese Medicine

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