Optimization of Bio-Solid Bidirectional Fermentation Conditions of  for Isatidis Radix by Response Surface Methodology

ZHANG Jing; GUO Ming-ye; CHENG Yi-xiong; ZHOU Li; QI Cen-cong; SHI Xin-yuan

doi:10.11653/syfj2013220034

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Optimization of Bio-Solid Bidirectional Fermentation Conditions of for Isatidis Radix by Response Surface Methodology

更新时间：2024-01-04

- Optimization of Bio-Solid Bidirectional Fermentation Conditions of for Isatidis Radix by Response Surface Methodology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 22, Pages: 34-37(2013)
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- DOI：10.11653/syfj2013220034
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- Published：2013
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ZHANG Jing, GUO Ming-ye, CHENG Yi-xiong, et al. Optimization of Bio-Solid Bidirectional Fermentation Conditions of for Isatidis Radix by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 34-37.
DOI：

ZHANG Jing, GUO Ming-ye, CHENG Yi-xiong, et al. Optimization of Bio-Solid Bidirectional Fermentation Conditions of for Isatidis Radix by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 34-37. DOI： 10.11653/syfj2013220034.

摘要

目的: 优化槐耳/板蓝根双向发酵条件并确定发酵终点。方法: 以菌质多糖含量为响应值

采用响应面法考察温度、相对湿度、接种量对槐耳/板蓝根双向发酵条件的影响

同时确定槐耳/板蓝根菌质双向发酵终点。利用紫外分光光度法测定菌质多糖含量。结果: 槐耳板蓝根菌质多糖的最佳发酵条件为温度29.4 ℃

接种量0.826 mL·g-1

相对湿度75.5%

菌质多糖质量分数11.496%

与预测值(11.548%)偏差0.45%

二项式拟合复相关系数0.9826

所得回归模型拟合情况良好

达到设计要求;确定发酵终点20 d。结论: 响应面法可用于优选槐耳/板蓝根双向发酵条件

具有简便、预测性良好等优点。

Abstract

Objective: To optimize bio-solid bidirectional fermentation conditions of Trametes robiniophia for Isatidis Radix and determine fermentation terminal. Method: Dependent variable was the content of polysaccharides

while independent variables were temperature

moisture and inoculation amount

response surface methodology was used to optimize bio-solid bidirectional fermentation conditions;Then fermentation terminal was defined.The content of polysaccharides was determined by UV. Result: Optimal fermentation conditions was as following:temperature 29.4 ℃

moisture 75.5%

inoculation amount 0.826 mL·g-1

bias between the observed value(11.496%) and the predicted value(11.548%) of the content of polysaccharide was 0.45%

regression coefficients of binomial fitting complex model was as high as 0.982 6;Terminal of bio-solid fermentation was 20 d. Conclusion: Response surface methodology could be used to optimize bio-solid fermentation conditions of T. robiniophia for Isatidis Radix with good prediction.

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Related Institution

China Academy of Chinese Medical Sciences

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