CUI Ming-jun, XU Jian-rong, XU Xiu-xia, et al. Optimization of Ultrasonic-Assisted Alkali Extraction and Acid Precipitation Technology for Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 38-40.
DOI:
CUI Ming-jun, XU Jian-rong, XU Xiu-xia, et al. Optimization of Ultrasonic-Assisted Alkali Extraction and Acid Precipitation Technology for Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 38-40. DOI: 10.11653/syfj2013220038.
Optimization of Ultrasonic-Assisted Alkali Extraction and Acid Precipitation Technology for Total Flavonoids from
Objective: To optimize ultrasonic-assisted alkali extraction and acid precipitation technology of total flavonoids from Acalypha australis. Method: UV spectrophotometry was used to determine the content of total flavonoids with rutin as a control component.With yield of total flavonoids as index
based on single factor test
effects of solid-liquid ratio
pH of alkaline extraction solvent
temperature
acid precipitation pH and time on extraction technology were investigated by orthogonal test. Result: The best extraction technology was as following:solid-liquid ratio of 1: 20
pH of extraction solvent 11
extracting temperature of 60 ℃
acid precipitation pH of 2.5
and ultrasonic time of 90 min.Under these conditions
extraction amount of total flavonoids from A. australis was 24.11 mg·g-1
and its yield could reach 87.76%. Conclusion: This optimized technology was stable and feasible with high extraction rate
it could provide experimental basis for development and utilization of A. australis.