XIONG Tian-qin, ZHAO Yu-min, ZE Rendawa, et al. HPLC Characteristic Fingerprint of Jiagasong Decoction[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 60-63.
DOI:
XIONG Tian-qin, ZHAO Yu-min, ZE Rendawa, et al. HPLC Characteristic Fingerprint of Jiagasong Decoction[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 60-63. DOI: 10.11653/syfj2013220060.
HPLC Characteristic Fingerprint of Jiagasong Decoction
Objective: To establish HPLC characteristic fingerprint of Jiagasong decoction. Method: Taking 6-gingerol and dehydrodiisoeugenol as control components
which were derermined by HPLC
chromatographic conditions were as follows:Platisil ODS column(4.6 mm×250 mm
5 μm)
mobile phase of acetonitrile-methanol-0.3% phosphoric acid solution for gradient elution
detection wavelength 274 nm
column temperature 25 ℃
flow rate 1.0 mL·min-1
injection volume 10 μL.Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2004A) was used in analysis 10 batches of Jiagasong decoction. Result: HPLC characteristic fingerprint of Jiagasong decoction was established
15 common peaks were received
two of them were identified as 6-gingerol and dehydrodiisoeugenol.Similarity of fingerprint among 10 batches of samples was more than 0.9
indicating a good correlation and basically the same chemical compositions. Conclusion: This assay method was simple
sensitive and accurate
this paper could provide scientific basis for quality evaluation of Jiagasong decoction.
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Related Author
WU Mei
A Ping
ZHOU Xue-shan
TAN Rui
Xi-qin CHEN
Zhi-lai ZHAN
Yang-yang LIU
Jian FENG
Related Institution
Hainan Provincial Key Laboratory of Resources Conservation and Development of Sourthern Medicine,Hainan Branch of Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College
National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences
School of Life and Health Sciences, Anhui Science and Technology University
College of Biotechnology, Tianjin University of Science & Technology