Objective: To establish an HPLC method for determining sodium danshensu and calycosin-7-glucoside in Yixin Tongmai mixtures. Method: HPLC conditions of sodium danshensu were as follows:Welchrom-C18 column(4.6 mm×250 mm

5 μm)

mobile phase of methanol-0.5% acetic acid(12: 88)

flow rate 1.0 mL·min-1

column temperature 30 ℃

injection volume 10 μL

detection wavelength 280 nm.But conditions of calycosin-7-glucoside were:mobile phase of acetonitrile(A)-0.2% formic acid solution(B) with gradient elution program(0-13 min

13%-15%A;13-23 min

15%-20%A;23-30 min

20%-40%A;30-40 min

40%A)

detection wavelength at 260 nm;Other conditions were the same as sodium danshensu. Result: Linear ranges of sodium danshensu and calycosin-7-glucoside were 0.213 44-5.336 and 0.078 72-2.361 6 μg

respectively;Average recoveries of them were 100.17%(RSD 2.02%) and 101.79%(RSD 1.40%)

respectively. Conclusion: This established HPLC method was rapid

accurate and reproducible

it could be used for quality control of Yixin Tongmai mixtures.