Objective: To establish a determination method of synephrine

norisoboldine and arecoline in Simo Tang oral liquid by HPLC. Method: HPLC method was set up

column was Agilent SCX

the mobile phase was methanol-0.2% phosphate solution

with the flow rate of 1.0 mL·min-1;the detection wavelength was set at 215 nm

column temperature was kept at 35 ℃. Result: Synephrine was linear within the range of 0.05-2.55 μg (r=0.999 9);the average recovery was 99.8%. Norisoboldine was linear within the range of 0.04-2.01 μg (r=0.999 9); the average recovery was 100.61%. Arecoline was linear within the range of 0.13-2.59 μg (r=0.999 8); the average recovery was 100.6%. Conclusion: The method is simple

accurate and stable

which is valuable to improve the quality standard of Simo Tang oral liquid.