Content Determination of Deoxynojirimycin in Silkworm Powder by RP-HPLC

ZHAO Yan-li; HUANG Yi-qi; HU Shan-mei; YANG Hui

doi:10.11653/syfj2013220124

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Content Determination of Deoxynojirimycin in Silkworm Powder by RP-HPLC

更新时间：2024-01-04

- Content Determination of Deoxynojirimycin in Silkworm Powder by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 22, Pages: 124-126(2013)
- 作者机构：
- 作者简介：
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- DOI：10.11653/syfj2013220124
  CLC：
- Published：2013
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ZHAO Yan-li, HUANG Yi-qi, HU Shan-mei, et al. Content Determination of Deoxynojirimycin in Silkworm Powder by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 124-126.
DOI：

ZHAO Yan-li, HUANG Yi-qi, HU Shan-mei, et al. Content Determination of Deoxynojirimycin in Silkworm Powder by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 124-126. DOI： 10.11653/syfj2013220124.

摘要

目的: 建立RP-HPLC测定家蚕中1-脱氧野尻霉素(DNJ)的含量。方法: 采用Hypersil NH2色谱柱(4.6 mm×250 mm

5 μm)

以乙腈-水(78:22)为流动相

流速1.0 mL·min-1

柱温30 ℃

检测波长203 nm。结果: 家蚕中DNJ在1.996~9.98 μg (r=0.999 6)峰面积与进样量呈良好的线性关系

平均加样回收率为98.4%。结论: 该方法前处理简单、实用

结果准确

可用于家蚕及制品中DNJ的质量控制。

Abstract

Objective: To establish assaying methods for the determination of deoxynojirimycin in silkworm. Method: The content of DNJ was determined by RP-HPLC

using a Hypersil NH2 (4.6 mm×250 mm

5 μm). The column temperature was maintained at 30 ℃. Acetonitrile-water (78: 22) was used as mobile phase;the flow-rate was 1.0 mL·min-1. The detecting wavelength was set at 203 nm. Result: The linear range was 1.996-9.98 μg (r=0.999 6). The average recovery was 98.4%. Conclusion: This method is simple

practical and accurate. It could be used for quality control of silkworm.

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Related Institution

Guangxi University of Chinese Medicine

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