HPLC Fingerprint and Chemical Pattern Recognition Method of

SU Su-jiao; LI Na; Chen Liang; LIN Pei-ling; DING Chun-hua; CHEN Shi-qiang; LIANG Yi-chi

doi:10.11653/syfj2013220144

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HPLC Fingerprint and Chemical Pattern Recognition Method of

更新时间：2024-01-04

- HPLC Fingerprint and Chemical Pattern Recognition Method of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 22, Pages: 144-147(2013)
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- DOI：10.11653/syfj2013220144
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- Published：2013
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SU Su-jiao, LI Na, Chen Liang, et al. HPLC Fingerprint and Chemical Pattern Recognition Method of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 144-147.
DOI：

SU Su-jiao, LI Na, Chen Liang, et al. HPLC Fingerprint and Chemical Pattern Recognition Method of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 144-147. DOI： 10.11653/syfj2013220144.

摘要

目的: 建立藤茶药材HPLC指纹图谱

并进行主成分聚类分析

为其质量控制提供依据。方法: 采用UltimateXB-C18色谱柱(4.6 mm×250 mm

5 μm);流动相乙腈-0.5%甲酸溶液梯度洗脱

检测波长365 nm;柱温40 ℃;应用SPSS软件对数据进行统计学分析。结果: 建立了藤茶药材HPLC指纹图谱共有模式

确定了11个共有峰

并指认了2个主要共有峰。通过主成分聚类分析

18个藤茶不同无性系种质可分为3类。结论: 该方法快速

可较全面的反映藤茶药材中化学成分的信息

为评价藤茶药材提供了科学实验依据。

Abstract

Objective: To establish an HPLC fingerprint of Ampelopsis grossedentata

and to make evaluation by principal component and cluster analysis for its quality control. Method: The HPLC analysis was performed on a UltimateXB-C18 column (4.6 mm×250 mm

5 μm) with column temperature at 40 ℃. The mobile phase was consisted of water containing 0.5% formic acid and acetonitrile in gradient mode. The detection wavelength was set at 365 nm. All data were analyzed with the SPSS statistical software. Result: The mutual mode of HPLC fingerprint has been established. There were 11 common peaks

two of which were identified. 18 samples from different clonal germplasm were classified into three groups based on principal component and cluster analysis. Conclusion: The method was time-saving and represent the chemical information and provide a scientific basis for quality control of Ampelopsis grossedentata.

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Related Author

JIANG Yong-mei

LI Gang

JIANG Feng-qin

HE Qun

WANG Gang

FENG Yang

CHEN Yu-mei

XIN Hua

Related Institution

The First Affiliated Hospital of Nanchang University

Key Laboratory of Modern Preparation of Traditional Chinese Medicine，Ministry of Education，Jiangxi University of Chinese Medicine

Key Laboratory of Modern Preparation of Traditional Chinese Medicine （TCM），Ministry of Education，Jiangxi University of TCM

College of Biological and Food Engineering，The Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of The Three Gorges Reservoir Area's Medicinal Herbs，Chongqing Three Gorges University

Chongqing Wanzhou Food and Drug Inspection Institute

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