LC-MS-MS Determination of Isofraxidan in Zhongjiefeng Fensanpian

LIANG Guo-hua; SU Shu-fen

doi:10.11653/syfj2013220148

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LC-MS-MS Determination of Isofraxidan in Zhongjiefeng Fensanpian

更新时间：2024-01-04

- LC-MS-MS Determination of Isofraxidan in Zhongjiefeng Fensanpian
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 22, Pages: 148-150(2013)
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- DOI：10.11653/syfj2013220148
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- Published：2013
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LIANG Guo-hua, SU Shu-fen. LC-MS-MS Determination of Isofraxidan in Zhongjiefeng Fensanpian[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 148-150.
DOI：

LIANG Guo-hua, SU Shu-fen. LC-MS-MS Determination of Isofraxidan in Zhongjiefeng Fensanpian[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 148-150. DOI： 10.11653/syfj2013220148.

摘要

目的: 建立高效液相色谱/串联质谱法测定肿节风分散片中异嗪皮啶。方法: 采用Agilent SB-C18色谱柱(2.1 mm×50 mm

1.8 μm)

流动相甲醇-水(35:65)

流速0.2 mL·min-1。三重四级杆质谱检测器

正离子多反应监测模式(MRM)

监测离子(m/z)222.9/189.7

222.9/161.9。结果: 异嗪皮啶在0.48~2.40 mg·L-1与峰面积具有良好的线性关系(r=0.999 2)

平均加样回收率为99.3%

RSD 0.55% (n=9)。结论: 方法简便易行

结果准确

重复性好

可用于肿节风分散片中异嗪皮啶的含量测定。

Abstract

Objective: To establish a method for determining isofraxidan in Zhongjiefeng Fensanpian by LC-MS-MS. Method: Agilent SB-C18(2.1 mm×50 mm

1.8 μm) column was used with methanol-H2O (35: 65) as mobilephase. The flow rate was 0.2 mL·min-1.MS-MS detection in multiple reactions monitoring (MRM) mode using positive electrospray ionization as the ionization source was applied to determine positive ions at (m/z)222.9/189.7

222.9/161.9. Result: Isofraxidan was linear in the range of 0.48-2.40 mg·L-1 (r=0.999 2). The average recovery was 99.3%

and RSD was 0.55% (n=9). Conclusion: The method is simple

rapid

accurate and reliable

which can be used for determination of isofraxidan in Zhongjiefeng Fensanpian.

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