Objective: To establish a method for simultaneous determination of cryptotanshinone

tanshinone I tanshinone ⅡA in Salviae Miltiorrhizae Radix et Rhizoma by dual-wavelength HPLC. Method: The Agilent ZORBAX SB-C18 column(4.6 mm×250 mm

5 μm)was used with a mobile phase of methanol-water at 1 mL·min-1

and the detection wave-lengths were set at 245 nm(tanshinone I)and 269 nm(cryptotanshinone and tanshinone ⅡA). Result: All calibration curves showed good linear regression in test concentration ranges(r>0.999 7)

and the overall average recoveries were in the range of 98.0%-100.1%(n=6) with RSD no more than 3.0%. Conclusion: The results indicated that this dual-wavelength HPLC method is rapid

accuracy

and simple

which is suitable for the quality control of the main phenanthraquinone in Salviae Miltiorrhizae Radix et Rhizoma.