Objective: To explore the protective of Trichosanthis Fructus and Allium Macrostemonis Bulbus on myocardial ischemic injury and its mechanism in rats. Method: Sprague Dawley rats were divided into 6 groups.The models of chronic myocardial ischemia was established by intraperitoneal injection of isoproterenol. After two weeks

activity of superoxide dismutase (SOD)

levels of lactate dehydrogenase (LDH)

creatine kinase (CK) and malondialdehyde (MDA) were measured.Western blotting was used to assay effects of Trichosanthis Fructus and Allii Macrostemonis Bulbus on mitogen-activated protein kinase (MAPKs) family of 3 major signaling molecule c-Jun n-Terminal kinase (JNK)

extracellular signal-regulated kinase (ERK)

phosphorylation of p38. Result: Compared with normal group

LDH

CK and MDA of model group increased significantly

SOD decreased significantly(P<0.05

P<0.01). Compared with normal group

P-p38 P-JNK P-ERK of model group was significantly increased (P<0.05

P<0.01). TKAM can significantly decrease the content of MDA

CK

LDH in serum.P-p38

P-JNK

P-ERK

MAPK protein levels of ischemia myocardial tissue increased significantly

TKAM can effectively lower levels of P-p38

P-JNK

P-ERK. Conclusion: Trichosanthis Fructus and Allium Macrostemonis Bulbus has a good protective effect on myocardial ischemia injury. The mechanism may be adopted on scavenging oxygen free radicals

reduce the oxidation inhibition of p38 and JNK phosphorylation of proteins in order to reduce ischemic injury.