Objective: The monoamine oxidase (MAO) activity screening model is established to guide the separation of Trionycis Carapax active peptides

and inspect the activity of the monomer. Method: Pargyline was used as positive drug with reference to the method of MAO kit to establish the monoamine oxidase activity screening model;the ethanol concentration of Trionycis Carapax water extraction liquid was adjusting to 20%

40%

60%

80%

60% in turn to get the alcohol precipitated parts

then screen the alcohol sink parts in this model

and continue the separation of optimal selection activity part

finally MAO inhibitory activity of the monomer was tested in the model. Result: The pargyline's concentration was 1.0 g·L-1

the inhibition rate on MAO was remarkable

which could amount to 98.30%-103.05%;when the concentration was at 1.0 g·L-1

the inhibitory rate of Trionycis Carapax water extraction liquid part on MAO was (23.34±9.66)%

20% alcohol precipitated part was (28.16±5.78)%

40% alcohol precipitated part was (30.69±7.17)%

60% alcohol precipitated part was (41.60±8.03)%

80% alcohol precipitated part was (64.91±2.94)%

80% alcohol precipitated part was(53.34±7.76)%.We get the Trionycis Carapax peptide from the 90% alcohol precipitated part. The Trionycis Carapax peptide's inhibition rate on MAO was obvious when the concentration ranged from 0.1-1.6 g·L-1

which ranged from 15.30% to 54.84%. Conclusion: The pargyline's inhibitory effect on MAO is remarkable

which suggests the model is successful and can guide separation of Trionycis Carapax

which has obvious inhibitory activity on MAO.