Effect of Genisteinon Inflammatory Cytokines Levels in Cerebral Ischemia/Reperfusion Injury

XUE Jing-hua; WANG Wan-hui; HUANG Jing-yao; LI Xiao; HUANG Zhi-hua; LI Liang-dong; ZENG Jing

doi:10.11653/syfj2013220197

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Effect of Genisteinon Inflammatory Cytokines Levels in Cerebral Ischemia/Reperfusion Injury

更新时间：2024-01-04

- Effect of Genisteinon Inflammatory Cytokines Levels in Cerebral Ischemia/Reperfusion Injury
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 22, Pages: 197-200(2013)
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- DOI：10.11653/syfj2013220197
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- Published：2013
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XUE Jing-hua, WANG Wan-hui, HUANG Jing-yao, et al. Effect of Genisteinon Inflammatory Cytokines Levels in Cerebral Ischemia/Reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 197-200.
DOI：

XUE Jing-hua, WANG Wan-hui, HUANG Jing-yao, et al. Effect of Genisteinon Inflammatory Cytokines Levels in Cerebral Ischemia/Reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 197-200. DOI： 10.11653/syfj2013220197.

摘要

目的: 研究染料木素对脑缺血再灌注损伤的保护作用

并初步探讨其保护作用与炎症反应之间的关系。方法: 雄性SD大鼠随机分成5组:假手术组、脑缺血再灌注损伤模型组、脑缺血再灌注损伤模型+溶剂对照组、脑缺血再灌注损伤模型+0.75 μmol·kg-1 染料木素组、脑缺血再灌注损伤模型+1.5 μmol·kg-1 染料木素组。采用大鼠大脑中动脉栓塞制作局灶性脑缺血再灌注损伤模型

缺血后10 min

于背部皮下注射不同剂量的染料木素、溶剂或生理盐水。缺血2 h

再灌注24 h后

麻醉后取脑

5-氯化三苯基四氮唑(triphenyl tetrazoliu chloride

TTC)染色

测定脑梗死体积;取前脑称湿重

烘干后称干重

计算脑组织含水率;化学比色法测定血清、脑组织乳酸脱氢酶(lactate dehydrogenase

LDH)活性;放射免疫学方法检测血清白介素-6(imerleukin-6

IL-6)、白介素-1β(imerleukin-1β

IL-1β)及肿瘤坏死因子-α(tumor necrosis factor-α

TNF-α)水平。结果: 与假手术组相比

模型组大鼠脑组织出现明显的白色梗死区

脑组织含水率升高(P<0.05)、LDH活性降低(P<0.05)

血清LDH活性升高(P<0.01)

血清IL-6(P<0.001)、IL-1β(P<0.01)及TNF-α(P<0.05)含量升高;与模型组相比

染料木素治疗组脑梗死范围减小

脑组织含水率降低(P<0.05)

血清LDH活性降低(P<0.05)

脑组织LDH活性升高(P<0.05)

血清IL-6(P<0.001)、IL-1β(P<0.05)及TNF-α(P<0.01)含量降低。结论: 染料木素对脑缺血再灌注损伤具有一定的保护作用

其保护机制与降低炎性细胞因子IL-6

IL-1β及TNF-α的合成进而减轻脑水肿有关。

Abstract

Objective: To explore the protective effects of genistein(Gen) on cerebral ischemia/reperfusion(I/R) injury and mechanism about inflammatory reaction and cytokine secretion. Method: A rat I/R injury was made through embolization of the middle cerebral artery

and rats were treated with Gen (0.75

1.5 μmol·kg-1)via sublingual vein injection. The volumes of infarction and water content in the cerebral tissue

the activities of lactate dehydrogenase (LDH) in serum and brain tissue

and the imerleukin(IL)-6

IL-1β and tumor necrosis factor-α(TNF-α) in the serum were measured. Result: Compare with the sham operation group

the rats in I/R group showed an obvious white infarction area

an increased water content(P<0.05)

and decrease LDH activity(P<0.05) in brain tissue. Meanwhile

it showed advances LDH activity(P<0.01) and IL-6(P<0.001). IL-1β(P<0.01) and TNF-α(P<0.05) levels in serum. After treated with Gen

the rats with cerebral I/R injury showed decreases in the volumes of infarction

water content (P<0.05)

LDH activity (P<0.05)

and IL-6(P<0.001)

IL-1β (P<0.05) and TNF-α (P<0.01) levels in serum

and an increased in LDH activity in brain tissue (P<0.05)). Conclusion: Genistein has a therapeutic effect on rat with cerebral I/R injury because its down-regulating inflammatory cytokines levels such as IL-1β

TNF-α

IL-6

suppresses inflammatory reaction

and then controls cerebral edema.

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