Antitumor Effects and Mechanism of Essential Oil of  and on Lung Cancer Cell Line A549

MU Jie; HUANG Xiao-qian; ZHU Ying; HE Song-min; FENG Bing; LI Xiu-li; ZHENG Guang-juan

doi:10.11653/syfj2013220209

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Antitumor Effects and Mechanism of Essential Oil of and on Lung Cancer Cell Line A549

更新时间：2024-01-04

- Antitumor Effects and Mechanism of Essential Oil of and on Lung Cancer Cell Line A549
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 22, Pages: 209-212(2013)
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- DOI：10.11653/syfj2013220209
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- Published：2013
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MU Jie, HUANG Xiao-qian, ZHU Ying, et al. Antitumor Effects and Mechanism of Essential Oil of and on Lung Cancer Cell Line A549[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 209-212.
DOI：

MU Jie, HUANG Xiao-qian, ZHU Ying, et al. Antitumor Effects and Mechanism of Essential Oil of and on Lung Cancer Cell Line A549[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 209-212. DOI： 10.11653/syfj2013220209.

摘要

目的: 探讨白术精油对人肺癌A549细胞的抑瘤作用及作用机制。方法: 白术精油乳剂灌胃SD大鼠

10日后采集含药血清体外培养A549细胞

噻唑蓝法(MTT)测其细胞生长抑制率

流式细胞术(FCM)检测白术精油含药血清对A549细胞细胞周期的影响

酶联免疫法(ELISA)检测白术精油含药血清对A549细胞半胱天冬蛋白酶3(caspase-3)蛋白表达的影响。结果: 白术精油中剂量组(150 μL·kg-1)15%含药血清、高剂量组(300 μL·kg-1)15%含药血清能够显著抑制A549细胞增殖

使A549细胞周期停滞在G0/G1期

并能显著增加A549细胞caspase-3的蛋白含量。结论: 白术精油具有抑制A549细胞增殖的作用

其抑瘤机制可能是控制A549细胞周期停滞在G0/G1期、促进细胞内caspase-3的蛋白表达促进细胞凋亡

从而抑制A549细胞增殖。

Abstract

Objective: To investigate the antitumor effect and the mechanism of Atractylodes macrocephala essential oils on human lung cancer A549 cells. Method: A. macrocephala oil emulsion was orally given to rats

after 10 days collection containing drug serum was collected

A549 cells were cultured in serum

MTT method was used to measure the activity of A549 cells

and ELISA method was used to assay the expression of caspase-3 in A549 cells. Result: 150

300 μL·kg-1 of containing A. macrocephalae oil 15% serum could significantly inhibit the proliferation of A549 cell

A549 cell cycle arrested in G0/G1 phase

and increased the content of caspase-3 protein in A549 cells. Conclusion: A.macrocephalae oil can inhibit the proliferation of A549 cells

its antitumor mechanism may control A549 cell cycle arrest in the G0/G1 phase

promote the expression of the caspase-3 protein.

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