Polysaccharide Isolated from Yulangsan Inhibits Duck Hepatitis B Virus and Protects Hepatocytes

ZUO Qiao-yun; TAO Li-qun; LUO Xiu; GE Wen-yi; HUANG Ren-bin

doi:10.11653/syfj2013220222

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Polysaccharide Isolated from Yulangsan Inhibits Duck Hepatitis B Virus and Protects Hepatocytes

更新时间：2024-01-04

- Polysaccharide Isolated from Yulangsan Inhibits Duck Hepatitis B Virus and Protects Hepatocytes
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 22, Pages: 222-226(2013)
- 作者机构：
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- DOI：10.11653/syfj2013220222
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- Published：2013
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ZUO Qiao-yun, TAO Li-qun, LUO Xiu, et al. Polysaccharide Isolated from Yulangsan Inhibits Duck Hepatitis B Virus and Protects Hepatocytes[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 222-226.
DOI：

ZUO Qiao-yun, TAO Li-qun, LUO Xiu, et al. Polysaccharide Isolated from Yulangsan Inhibits Duck Hepatitis B Virus and Protects Hepatocytes[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(22): 222-226. DOI： 10.11653/syfj2013220222.

摘要

目的: 观察玉郎伞多糖(YLSP) 在鸭乙型肝炎病毒(DHBV)持续性感染模型中对鸭血清乙型肝炎病毒DNA的抑制作用及保护肝细胞的作用。方法: 将DHBV-DNA强阳性麻鸭随机分为YLSP高、中、低剂量组(10

2.5 g·kg-1)、拉米夫定(3TC

0.05 g·kg-1) 组和模型组

以上各组每日上午灌胃给药 l 次

连续 14 d。于用药前(T0) 、用药7 d (T7)和14 d (T14)及停药后3 d (P3)取静脉血用实时荧光定量PCR检测DHBV-DNA含量

同时采用酶联免疫吸附(ELISA)法测定上清液鸭乙型肝炎病毒表面抗原(DHBsAg)和e抗原(DHBeAg)的滴度;在停药3 d后

取肝脏匀浆

检测肝匀浆液中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性以及丙二醛(MDA)、谷胱甘肽(GSH)的含量。结果: 与模型组相比

YLSP高、中剂量治疗组给药7 d (T7)和14 d (T14)即能明显抑制DHBV-DNA复制及血清DHBsAg

DHBeAg的滴度显著降低(P<0.05或P<0.01)

停药后3 d (P3)

YLSP高剂量组仍能显示出持续有效

血清DHBV-DNA水平及血清DHBsAg

DHBeAg的滴度均无反跳现象 (P<0.05或P<0.01);在停药3d后

肝匀浆液中SOD

GSH-Px活性以及MDA

GSH的含量

YLSP高、中剂量组仍能显示出持续有效

没有出现反跳现象(P<0.05或P<0.01)。结论: YLSP具有有效抑制DHBV-DNA和保护肝细胞的作用。

Abstract

Objective: To study the inhibitory effect of Yulangsan polysaccharide (YLSP) on duck hepatitis B virus (DHBV) persistent infection in the model for the serum DHBV-DNA and the protecting effect on hepatocytes. Method: Ducklings were infected with DHBV and randomly divided the 3YLSPS dose groups(10

2.5 g·kg-1)

lamivudine (3TC

0.05 g·kg-1) and model groups respectively

continuous administration for 14 days. The serum content of DHBV-DNA was determined by real time fluorescent quantity polymerase chain reaction(FQ-PCR) method

DHBsAg and DHBeAg in serum were detected by ELISA before treatment and on the 7th

14th day after treatment and At 3 days after stopping treatment

took the liver homogenate and detected in liver and serum superoxide dismutase(SOD)

glutathione peroxidase(GSH-Px)activity and malondialdehyde(MDA)

L-glutathione (GSH) content. Result: Compared with modle group

the serum levels of DHBV-DNA

DHBsAg and DHBeAg decreased significantly 7 and 14 days after treatment with high-dose and middle-dose YLSP and at 3 days after stopping treatment the DHBV-DNA

DHBsAg and DHBeAg in serum in high-dose group were not rebounded

in liver and serum SOD

GSH-Px activity and MDA

GSH content

high-dose and middle-dose YLSP could still show a sustained and effective

no rebound phenomena (P < 0.05 or P < 0.01). Conclusion: YLSP can inhibit replication of DHBV and protect hepatocytes.

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