Objective: To investigate the effect of compatibility of Astragalus and different dosage of Radix Bupleuri on the model of spleen-qi deficiency with fever in rats. Method: The male Wistar rats were assigned into the normal control group modle group

the spleen-qi deficiency with fever group (model group)

the Astragalus with high dosage Radix Bupleuri group

the Astragalus with medium dosage Radix Bupleuri group

the Astragalus with low dosage Radix Bupleuri group

and the only Radix Bupleuri group. The rats in model group and medicine groups were treated with excess fatigue plus out of constant diet for 21 days. From the 17th day

the medicine group rats were fed with 4.56

2.95

2.41 g·kg-1 and 2.41 g·kg-1 (Radix Bupleuri only) once a day and the other two groups were fed with physiological saline. On the 22th day

the rats in model group and medicine groups were respectively intraperitoneally injected with lipopolysaccharide(LPS

80 μg·kg-1) to induce fever.The rats of the normal group were injected with physiological saline.The appearance and behavior characteristics of rats were observed twice a week and the rectal temperature was measured at different time after ip LPS. The content of braingut petide in peripheral blood was also measured. Result: Compared with the normal group

the rectal temperature of the spleen-qi deficiency with fever group raised at each time point in different degree after ip LPS

especially at 60

220 min(P<0.05);the integration of appearance and behavior characteristics increased significantly(P<0.05);somatostatin(SS)

cholecystokinin-8(CCK-8)

β-endomorphin(β-EP) in peripheral blood increased significantly(P<0.05). Compared with model group

the rectal temperature of every medicine group were decreased significantly in different degree(P<0.05)

especially in the only Radix Bupleuri group and the Astragalus with low dosage Radix Bupleuri group

the temperature curve deviated from the normal group;the integration of appearance and behavior characteristics decreased significantly(P<0.05)only in the Astragalus with medium and low dosage Radix Bupleuri groups;serum β-EP increased significantly(P<0.05)in all medicine groups;serum CCK-8

SS showed a downward trend in the Astragalus with high

medium and low dosage Radix Bupleuri groups

but sexum CCK-8

SS in the only Radix Bupleuri group showed a rising trend. Compared with the only Radix Bupleuri group

serum SS decreased significantly(P<0.05) in the Astragalus with high

medium and low dosage Radix Bupleuri groups;serum β-EP decreased significantly(P<0.05) in the Astragalus with large dosage Radix Bupleuri group;serum CCK-8 decreased significantly(P<0.05)in the Astragalus with medium and low dosage Radix Bupleuri groups. Conclusion: The compatibility of Astragalus and different dosage of Radix Bupleuri has antipyretic effect on the model group and the integration of appearance and behavior characteristics and braingutpetide were improved in different degrees. The only Radix Bupleuri group has the antipyretic effect only. The Astragalus with low dosage Radix Bupleuri group has the best controlling effect for the comprehensive indices.