Optimization of Separation and Purification Technology for Total Flavonoids from Ziziphi Spinosae Semen by Macroporous Adsorption Resin

WANG Yong; LI Hong-fu; WEI Na; LI Yong-hui

doi:10.11653/syfj2013230004

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Optimization of Separation and Purification Technology for Total Flavonoids from Ziziphi Spinosae Semen by Macroporous Adsorption Resin

更新时间：2024-01-04

- Optimization of Separation and Purification Technology for Total Flavonoids from Ziziphi Spinosae Semen by Macroporous Adsorption Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 23, Pages: 4-7(2013)
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- DOI：10.11653/syfj2013230004
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- Published：2013
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WANG Yong, LI Hong-fu, WEI Na, et al. Optimization of Separation and Purification Technology for Total Flavonoids from Ziziphi Spinosae Semen by Macroporous Adsorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(23): 4-7.
DOI：

WANG Yong, LI Hong-fu, WEI Na, et al. Optimization of Separation and Purification Technology for Total Flavonoids from Ziziphi Spinosae Semen by Macroporous Adsorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(23): 4-7. DOI： 10.11653/syfj2013230004.

摘要

目的: 优选大孔树脂分离纯化酸枣仁醇提物中总黄酮的工艺。方法: 通过静态和动态吸附-洗脱试验比较6种不同型号大孔树脂对酸枣仁总黄酮的吸附和解吸性能，筛选最佳大孔树脂型号;以总黄酮质量浓度为指标，采用单因素试验考察药液质量浓度、吸附速率、上样体积、洗脱剂浓度、洗脱速率等因素对纯化工艺的影响。结果: 大孔树脂分离纯化酸枣仁总黄酮的最佳工艺条件为选择SP-207型大孔树脂，上样液质量浓度0.5 g·mL-1，吸附速率1 BV·h-1，上样量5 BV，加70%乙醇4.5 BV以4 BV·h-1的速率洗脱;酸枣仁总黄酮纯度达65.47%。结论: 该优选工艺稳定可行，适用于酸枣仁总黄酮的分离纯化。

Abstract

Objective: To optimize separation and purification technology of total flavonoids from ethanol extract of Ziziphi Spinosae Semen(ZSS) by macroporous adsorption resin. Method: Adsorption and desorption properties of six different types of macroporous resins for total flavonoids from ZSS were compared by static and dynamic adsorption and desorption tests to select optimum type of macroporous resin;With the content of total flavonoids as index

single factor tests were adopted to investigate adsorption and elution conditions of macroporous resin

such as the concentration of sample diluted

adsorption rate

eluent concentration and so on. Result: SP-207 macroporous resin was adopted

optimum separation and purification conditions for total flavonoids from ZSS were as follows:the concentration of sample solution 0.5 g· mL-1

adsorption rate 1 BV· h-1

sample volume 5 BV

eluted by 4.5 BV of 70% ethanol at a flow rate of 4 BV· h-1;The content of total flavonoids from extract of ZSS was 65.47%. Conclusion: This optimized process was stable

feasible and suitable for separation and purification of total flavonoids from ZZS.

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Related Institution

Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine

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