Optimization of Separation and Purification Technology for Total Flavonids from Leaves of  by Macroporous Resin

PAN Xiao-wei; HUANG Yi-ping

doi:10.11653/syfj2013230039

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Optimization of Separation and Purification Technology for Total Flavonids from Leaves of by Macroporous Resin

更新时间：2024-01-04

- Optimization of Separation and Purification Technology for Total Flavonids from Leaves of by Macroporous Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 23, Pages: 39-42(2013)
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- DOI：10.11653/syfj2013230039
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- Published：2013
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PAN Xiao-wei, HUANG Yi-ping. Optimization of Separation and Purification Technology for Total Flavonids from Leaves of by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(23): 39-42.
DOI：

PAN Xiao-wei, HUANG Yi-ping. Optimization of Separation and Purification Technology for Total Flavonids from Leaves of by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(23): 39-42. DOI： 10.11653/syfj2013230039.

摘要

目的: 优选楮叶总黄酮的大孔树脂纯化工艺。方法: 以楮叶总黄酮为指标，通过大孔树脂静态吸附和动态吸附试验筛选树脂型号，通过单因素试验考察上样液质量浓度、洗脱剂用量、洗脱流速等因素对纯化工艺的影响。结果: HPD450型大孔树脂分离效果最好，其最佳工艺参数为上样液质量浓度4.4 g·L-1，径高比1∶8，上样量6 BV，吸附速度1 BV·h-1，加5 BV水洗除杂，用50%乙醇8 BV以1 BV·h-1的速度洗脱。总黄酮纯度由精制前的30.65%提高至75.75%，总黄酮保留率达79.89%，除杂率达64.88%。结论: HPD450型大孔树脂纯化楮叶总黄酮的效果良好，且工艺合理稳定，可推广于工业生产中应用。

Abstract

Objective: To optimize purification technology conditions of total flavonoids from leaves of Broussonetia papyrifera by macroporous adsorption resin. Method: With total flavonoids from leaves of B. papyrifera as index

type of macroporous resin was screened by by static adsorption-desorption and dynamic adsorption-desorption tests

effects of eluent consumption

elution flow rate

the concentration of sample solution and other factors on purification technology were investigated by single factor tests. Result: HPD450 was selected for its excellent adsorption and desorption properties

optimum process was as following:the concentration of sampling solution 4.4 g· L-1

sampling velocity 1 BV· h-1

sample liquid volume 6 BV

eluted by 5 BV deionized water and 8 BV 50% ethanol at speed of 1 BV· h-1

diameter to height ratio of resin 1:8

collected eluate.Under these conditions

purity of total flavonoids was up to 75.75% from 30.65%

retention rate of total flavonoids reached 79.89%

impurity removal rate reached 64.88%. Conclusion: HPD450 macroporous resin was suitable for separation and purification of total flavonoids from leaves of b. papyrifera

optimized purification technology was stable and feasible

it could be extended for industrial production applications.

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Related Author

FU Yan-li

SONG Hong

TONG Zhi-ping

WANG Si-wen

ZENG Zheng

ZHOU Xu

WANG Yong

LI Hong-fu

Related Institution

Affiliated Hospital of Jiangsu University

School of Pharmacy,Jiangsu University

Pharmacy Technology Center，Tsinghua University

Center of Pharmacy R&D，XinJiang Huachun Bio-Pharmaceutical Co. Ltd.

School of Life Sciences， Tsinghua University

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