ZHOU Xiang-long, GAO Xiao-li, DING Nan, et al. Optimization of Purification Technology for Total Polyphenols from Acid Granti Pericarpium in Kashi[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(23): 54-58.
DOI:
ZHOU Xiang-long, GAO Xiao-li, DING Nan, et al. Optimization of Purification Technology for Total Polyphenols from Acid Granti Pericarpium in Kashi[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(23): 54-58. DOI: 10.11653/syfj2013230054.
Optimization of Purification Technology for Total Polyphenols from Acid Granti Pericarpium in Kashi
Objective: To optimize purification process of total polyphenols from acid Granti Pericarpium in Kashi. Method: With the content of total polyphenols as index
type of macroporous resin was screened by static adsorption-elution test
single factor tests were adopted to optimize purification process of total polyphenols by taking sample solution concentration
eluent consumption
adsorption speed and others as factors. Result: HPD500 macroporous resin was selected
optimum purification process was as follows:the crude drug concentration of sample solution 20.8 g· L-1
pH of sample solution 2
sample volume 8 BV
adsorption rate 2 BV· h-1
adsorption time 12 h
eluted impurities with 7 BV of distilled water and 8 BV of 50% ethanol
collected ethanol eluate;Purity of total polyphenols was up to 66.93% from 24% before purification
yield of extract was 34.80%. Conclusion: HPD500 macroporous resin was suitable for purification of total polyphenols from acid Granti Pericarpium
optimized process conditions were simple and feasible with high efficiency and low toxicity.