Objective: To investigate determination of total iridoid glycoside

total flavonoids and total phenethanol glycosides from different extractive of extract of Lamiophlomis rotata. Method: Water extract of L. rotata were separated and enriched obtain water eluate and 70% ethanol solution eluateⅠ(70% ethanol solution eluted polyamide column)

70% ethanol solution eluate Ⅱ (70% ethanol solution eluting macroporous resin column) by polyamide and macroporous resin. Total iridoid glycosides ingredients were assayed by the first derivative spectrophotometry;the total flavonoids and total benzene glycosides were assayed by dual wavelength UV spectrophotometry

aluminum nitrate-sodium nitrite UV spectrophotometry. Result: Recoveries of the total iridoid glycosides was 104.40%

RSD was 0.64% at 252 nm wavelength by the first derivative UV spectrophotometry. Recoveries of the total flavonoids were 96.23% and 104.48%

RSDs were 7.27% and 3.61% at the 268 nm and 363 nm wavelengths by UV spectrophotometry. Recoveries of the total benzene glycosides was 100.26%

RSD was 2.92% at 332 nm wavelength. Conclusion: The first derivative ultraviolet spectrophotometry can determine the total iridoid glycosides. The ultraviolet spectrophotometry can determine the total flavonoids and total benzene glycosides.