Study on the Quality Standard for Processed

LI De-bin; HUANG Zhi-fang; LIU Yun-hua; LIU Yu-hong; CHEN Yan; YI Jin-hai

doi:10.11653/syfj2013230146

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Study on the Quality Standard for Processed

更新时间：2024-01-04

- Study on the Quality Standard for Processed
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 23, Pages: 146-150(2013)
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- DOI：10.11653/syfj2013230146
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- Published：2013
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LI De-bin, HUANG Zhi-fang, LIU Yun-hua, et al. Study on the Quality Standard for Processed [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(23): 146-150.
DOI：

LI De-bin, HUANG Zhi-fang, LIU Yun-hua, et al. Study on the Quality Standard for Processed [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(23): 146-150. DOI： 10.11653/syfj2013230146.

摘要

目的: 建立炮天雄的质量标准。方法: 采用薄层色谱法对苯甲酰新乌头原碱进行定性研究;采用高效液相色谱方法，选用Eclipse XDB-C18色谱柱（4.6 mm×250 mm，5 μm），柱温35℃，流动相A为0.1 mol·L-1醋酸铵溶液（每1 000 mL加冰醋酸0.5 mL），B为四氢呋喃-乙腈（15∶25），梯度洗脱，体积流量1 mL·min-1，检测波长235 nm。结果: 炮天雄中水分为10.1%~12.3%;定性鉴别专属性强;苯甲酰新乌头原碱、苯甲酰乌头原碱、苯甲酰次乌头原碱、新乌头碱、次乌头碱和乌头碱分别在0.049 4~1.976，0.023 32~0.932 8，0.049 95~1.998，0.024 46~0.978 56，0.024 91~0.996 48，0.022 02~0.880 8 μg进样量呈良好线性关系，r均为0.999 9;平均回收率分别为98.79%，100.66%，97.06%，97.91%，103.58%，102.77%;RSD分别为1.90%，2.23%，1.91%，1.96%，1.08%，2.19%。结论: 该方法操作简便，结果准确，可作为炮天雄的质量控制方法。

Abstract

Objective: To establish the quality standards for Chinese medicine processed Aconitum carmichneli (Tuber of Aconitum armichaeli). Method: Benzoylmesaconine was identified by TLC method. Monoester alkaloids and diester type alkaloids were determined by HPLC method. HPLC condition was as follows:The Eclipse XDB-C18 (4.6 mm×250 mm

5 μm) column was used;the column temperature was maintained at 35℃. The mobile phase system was 0.1 mol· L-1 ammonium acetate (per 1 000 mL contains 0.5 mL glacial acetic acid

phase A) and tetrahydrofuran-acetonitrile (15:25

phase B) with gradient elution. The flow rate was 1 mL· min-1. The detection wavelength was set at 235 nm. Result: The water content was ranged from 10.1% to 12.3%. The qualitative identification was specific. Benzoylmesaconine

benzoylaconine

benzoylhypaconine

mesaconitine

hypaconitine and aconitine showed good linear relationship in the range of 0.049 4-1.976

0.023 32-0.932 8

0.049 95-1.998

0.024 46-0.978 56

0.024 91-0.996 48

0.022 02-0.880 8 μg respectively (r=0.999 9 for all six substances). The average recovery rates of benzoylmesaconine

benzoylaconine

benzoylhypaconine

mesaconitine and hypaconitine were 98.79%

100.66%

97.06%

97.91%

103.58% and 102.77% respectively with RSD of 1.90%

2.23%

1.91%

1.96%

1.08% and 2.19% respectively. Conclusion: The method is simple

reproducible and accurate;it can be used as the quality control methods of the Chinese medicine processed Aconitum carmichaeli.

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