Objective: To investigate the effects and possible mechanisms of EtOAc extract from Gastrodia elata on oxidative damage of induced by H2O2 in PC12 cells. Method: PC12 cells were cultured and exposed to H2O2(50-100 μmol· L-1)for 2 h to establish oxidative damage model. The protective effect of EtOAc extract was observed by morphological identification

colorimetric MTT assay

leakage rate of lactic dehydrogense(LDH)

intracellular reactive oxygen species(ROS) level and total superoxide dismutase(T-SOD) activity. Result: Compared with the model group

EtOAc extract from G. elata at 20 mg· L-1 could improve the cell morphology significantly

increase the survival rate of 14.7% (P<0.01);EtOAc extract at 20

40

80 mg· L-1 decrease the LDH release of 22.0%

29.3%

20.2% (P<0.01) from cells into culture medium

raise intracellular T-SOD acivity (P<0.01);EtOAc extract at 80 mg· L-1 decrease the ROS level (P<0.01). Conclusion: EtOAc extract from G. elata has a obvious antioxidant effect in vitro.