Study on the Anti-inflammatory Mechanism of Sanhuangfang Compound

LI Miao; YIN Hua; ZHANG Cheng-long

doi:10.11653/syfj2013230213

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Study on the Anti-inflammatory Mechanism of Sanhuangfang Compound

更新时间：2024-01-04

- Study on the Anti-inflammatory Mechanism of Sanhuangfang Compound
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 23, Pages: 213-215(2013)
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- DOI：10.11653/syfj2013230213
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- Published：2013
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LI Miao, YIN Hua, ZHANG Cheng-long. Study on the Anti-inflammatory Mechanism of Sanhuangfang Compound[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(23): 213-215.
DOI：

LI Miao, YIN Hua, ZHANG Cheng-long. Study on the Anti-inflammatory Mechanism of Sanhuangfang Compound[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(23): 213-215. DOI： 10.11653/syfj2013230213.

摘要

目的: 观察三黄方对脂多糖（LPS）诱导的小鼠巨噬细胞RAW264.7分泌细胞因子的影响，探讨其抗炎作用机制。方法: CCK-8法测定大黄、黄芩、黄柏及三黄方对RAW264.7细胞的毒性作用，酶联免疫吸附试验（ELISA）检测细胞上清液中的一氧化氮（NO）、白介素-1β（IL-1β）、前列腺素2（PGE2）、肿瘤坏死因子-1α（TNF-1α）的含量。结果: 三黄方可抑制LPS诱导的RAW264.7细胞释放细胞炎症因子NO，IL-1β，PGE2，TNF-1α的量，且三黄方的作用均强于各药单独使用。结论: 三黄方及方中大黄、黄芩、黄柏在浓度范围100~1.562 5 mg·L-1对RAW264.7细胞无显著毒性。三黄方通过抑制细胞因子NO，IL-1β，PGE-2，TNF-1α释放发挥其抗炎作用的。

Abstract

Objective: To investigate the effect of Sanhuangfang compound on lipopolysaccharide (LPS)-induced production of inflammatory cytokine in RAW264.7 cell. Method: The cytotoxicity of Rheum palmatum

Scutellaria baicalensis

Phellodendron chinense and Sanhuangfang compound on RAW264.7 cells was detected by CCK8 method;Levels of nitric oxide(NO)

interleukin(IL )-1β

prostaglandin E2(PGE2) and tumor necrosis factor-α(TNF-α) in supernatant were assayed by ELISA. Result: Sanhuangfang compound inhibited the secretion of NO

IL-1β

PGE2 and TNF-α in LPS-induced RAW264.7 cells. Conclusion: Rheum palmatum

Scutellaria baicalensis

Phellodendri chinensis and Sanhuangfang compound in 100-1.562 5 mg·L-1 concentration has non-cytotoxic macrophages. The anti-inflammatory effects of Sanhuangfang compound may be mediated in part by inhibiting the production of inflammatory mediators.

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Related Institution

Department of Pharmacy,Zhujiang Hospital,Southern Medical University

Nanjing University of Chinese Medicine,Key Laboratory for Modern Research of Traditional Chinese Medical Formulae of Jiangsu

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