Objective: To study the effect of Alpiniae Oxyphyllae Fructus on defecating function of normal mice and its possible mechanism

and the dry effects were characterized. Method: The SPF mice were divided into 5 groups: normal group

Alpiniae Oxyphyllae Fructus with different doses groups (20

10

5 g· kg-1)

Alpiniae Oxyphyllae Fructus(20 g· kg-1) and increase liquid soup(20 g·kg-1) combination group. All mice were orally administered once a day for 28 d. In the 7

14

21

28 d to observe the effects of defecation function to animals

and from the aspects of intestinal moisture

colonic electromyography to explain the mechanism that the dry effects of Alpiniae Oxyphyllae Fructus impacting on gut. Result: After 6 h observation

the first time of defecation with low dose was 142.7 min

dry manure was 10.1 grains

weight was 176.6 mg in 28 d; the first time of defecation with moderate dose was 145.4 min

dry manure was 10.2 grains

weight was 177.0 mg in 21 d; the first time of defecation with high dose was 160.3 min

dry manure was 9.7 grains

weight was 170.5 mg in 21 d. Alpiniae Oxyphyllae Fructus is able to make the normal animal appear symptoms of constipation gradually through dose-dependent and time-dependent. After administrated for 28 days

the moisture content of colon lumen of low dose

moderate dose and high dose group of Alpiniae Oxyphyllae Fructus was 66.23%

64.42%

61.53%

significantly reduce the moisture content of colon lumen; frequency of colonic electromyography of low dose

moderate dose and high dose group of Alpiniae Oxyphyllae Fructus was 43.40

45.63

48.26 times per minute

the amplitude of colonic electromyography was 66.23

64.42

61.53 mv

significantly faster frequency of colonic electromyography

reduce the amplitude of colonic electromyography.Increase liquid soup have obvious antagonism effects on above-mentioned constipation caused by Alpiniae Oxyphyllae Fructus. Conclusion: Dry effect characterization of Alpiniae Oxyphyllae Fructus can cause constipation of normal mice

and it's related to the promotion of intestinal water reabsorption and reduced intestinal transit capacity.