Objective: To investigate effect of Fu's cupping therapy on transdermal absorption of tetrahydropalmatine patches. Method: With composite score of AUC0-t

Tmax and Cmax as index

orthogonal design was adopted to optimize technology parameters of Fu's cupping therapy by taking tank methods

pressure and time as factors.UPLC-MS/MS was used to determine plasma concentration of tetrahydropalmatine in tetrahydropalmatine patches

mobile phase of acetonitrile-water(70:30)

flow rate 0.3 mL·min-1

autosampler temperature 30℃

column temperature 30℃

injection volume 3 μL

electrospray ionization(ESI+)

m/z 356.27

capillary voltage 3.38 kV

cone voltage 38 V

ion source temperature 150℃

desolvation temperature 350℃

anti-cone gas flow rate 50 L·h-1

desolvation gas flow rate 650 L·h-1

collision gas flow rate 0.16 mL·min-1. Result: The best conditions of Fu's cupping therapy was:scraping tank

low pressure (-0.02 MPa) and time of 15 min. Conclusion: Fu's cupping therapy could significantly improve transdermal absorption of tetrahydropalmatine patches

which was a new physical method for promoting transdermal absorption.