Optimization of Extraction Technology for Total Triterpene Acids from Abri Herba by Semi-Bionic Method

LAI Hong-fang; DENG Qiu-fen

doi:10.11653/syfj2013240046

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Optimization of Extraction Technology for Total Triterpene Acids from Abri Herba by Semi-Bionic Method

更新时间：2024-01-04

- Optimization of Extraction Technology for Total Triterpene Acids from Abri Herba by Semi-Bionic Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 24, Pages: 46-48(2013)
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- DOI：10.11653/syfj2013240046
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- Published：2013
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LAI Hong-fang, DENG Qiu-fen. Optimization of Extraction Technology for Total Triterpene Acids from Abri Herba by Semi-Bionic Method[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 46-48.
DOI：

LAI Hong-fang, DENG Qiu-fen. Optimization of Extraction Technology for Total Triterpene Acids from Abri Herba by Semi-Bionic Method[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 46-48. DOI： 10.11653/syfj2013240046.

摘要

目的：优选鸡骨草中总三萜酸的半仿生法提取工艺。方法：采用UV测定总三萜酸含量。以总三萜酸提取率为指标，在单因素试验基础上，通过正交试验考察提取温度、酸提取时间、碱提取时间、料液比对鸡骨草总三萜酸提取工艺的影响，并与传统水提法、超声波法进行比较。结果：最佳提取工艺条件为料液比1:25，恒温水浴温度100℃，酸（pH 2）提取时间55 min，碱（pH 8）提取时间25 min；总三萜酸提取率达0.123%，高于传统水提法（0.089%），但低于超声波法（0.304%）。结论：采用半仿生法提取鸡骨草中总三萜酸的工艺具有绿色环保、成本低等优点。

Abstract

Objective: To optimize semi-bionic extraction technology of total triterpene acids from Abri Herba. Method: The content of total triterpene acids was determined by UV.With yield of total triterpene acids as index

based on single factor tests

orthogonal test was adopted to optimize semi-bionic extraction technology of total triterpene acids by taking extraction temperature

ratio of solid-liquid

acid and alkali extraction time as factors

then compared with water extraction method and lower than ultrasonic method. Result: Optimum semi-bionic extraction technology was as following:extraction temperature at 100℃

solid-liquid ratio of 1:15

acid(pH 2) extraction time 55 min

alkali(pH 8) extraction time 25 min;Under these conditions

yield of total triterpene acids was 0.123%

which was higher than water extraction method(0.089%) and lower than ultrasonic method(0.304%). Conclusion: Optimized semi-bionic extraction process had advantages of green environmental protection

low cost and so on.

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Related Institution

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