MA Yan, ZHANG Ning-ning. Extraction of Total Flavonoids from Citri Reticulatae Pericarpium by Opposite Dual-Frequency Ultrasonic Method[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 49-52.
DOI:
MA Yan, ZHANG Ning-ning. Extraction of Total Flavonoids from Citri Reticulatae Pericarpium by Opposite Dual-Frequency Ultrasonic Method[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 49-52. DOI: 10.11653/syfj2013240049.
Extraction of Total Flavonoids from Citri Reticulatae Pericarpium by Opposite Dual-Frequency Ultrasonic Method
Objective: To optimize opposite dual-frequency ultrasonic extraction technology of total flavonoids from Citri Reticulatae Pericarpium. Method: Hydrophone method was adopted to investigate cavitation effect of single frequency and opposite dual-frequency ultrasonic under different frequency and power.With yield of total flavonoids as index
effects of extraction time
ethanol concentration
ultrasonic electric power and different frequency/power combinations on extraction technology were investigated by single factor tests. Result: Cavitation effect of opposite dual-frequency ultrasonic(20+25) kHz was greater than single frequency ultrasonic(frequency were 20 kHz and 25 kHz
respectively);Under a temperature of (20±0.5)℃
extraction time of 10 min
ethanol concentration of 60%
opposite dual-frequency ultrasonic combination of (20 kHz
25 kHz)
yield of total flavonoids was 4.52%
which was higher than the same extraction condition of single frequency ultrasonic(3.95%
4.03%). Conclusion: Opposite dual-frequency ultrasonic extraction for active ingredients from Chinese herbal medicines was an ideal method
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