Objective: To establish quantitative analysis of multi-components with a single-marker(QAMS) for determining contents of six ingredients from Gycyrrhizae Radix et Rhizoma. Method: Contents of six ingredients from Gycyrrhizae Radix et Rhizoma were determined by HPLC

mobile phase of acetonitrile(A)-0.1% phosphoric acid solution(B) with a gradient elution program(0-20 min

90%-68% B;20-45 min

68%-30% B;45-75 min

30%-5% B)

detection wavelength(0 min

275 nm;32 min

250 nm;33.5 min

300 nm;34.5 min

360 nm;53 min

280 nm;55 min

270 nm;57.5 min

265 nm).With glycyrrhizic acid as a internal standard

relative correlation factors(RCF) of liquiritin

liquiritiyenin

isoliquiritiyenin

glycyrrhetinic acid and licochalcone A to glycyrrhizic acid were calculated

contents of these six ingredients were determined by the external standard method and QAMS

respectively. Result: These two methods did not show significant difference in assay results of contents of these six ingredients with similarity of 0.999 9. Conclusion: QAMS could be used for quality control of Gycyrrhizae Radix et Rhizoma.